Recombinant Chymase 1, Mast Cell (CMA1) Mus musculus (Mouse) Recombinant protein

CYH; CYM; Alpha-chymase; Mast cell protease I

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 30.3kDa, Accurate 30kDa(Analysis of differences refer to the manual)
  • Residues & TagsLeu9~Asn247 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point9.9
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  • Recombinant Chymase 1, Mast Cell (CMA1) Packages (Simulation)
  • Recombinant Chymase 1, Mast Cell (CMA1) Packages (Simulation)
  • RPG515Mu01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Anti-Angiotensin and Hypoglycemic Treatments Suppress Liver Metastasis of Colon Cancer CellsKarger: 000330169
  • Diabetes-associated angiotensin activation enhances liver metastasis of colon cancerPubMed: 22552372
  • Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis HerpetiformisPubmed:25400334
  • Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgeryPubmed:25677271
  • The autocrine role of tryptase in pressure overload-induced mast cell activation, chymase release and cardiac fibrosisPubMed: 26722642
  • Oral sensitization to whey proteins induces age-and sex-dependent behavioral abnormality and neuroinflammatory responses in a mouse model of food …Pubmed:29685134
  • Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patientsPubmed: 30365640
  • Platelets trigger perivascular mast cell degranulation to cause inflammatory responses and tissue injuryPubmed: 32206714
  • Disruption in the balance between apolipoprotein A‐I and mast cell chymase in chronic hypersensitivity pneumonitisPubmed: 33016012
  • Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia SyndromePubmed:35612327

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