Recombinant Collagen Type IV Alpha 1 (COL4a1) Rattus norvegicus (Rat) Recombinant protein

COL4-A1; Arresten; Collagen Alpha-1(IV)chain

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 31.3kDa, Accurate 31kDa(Analysis of differences refer to the manual)
  • Residues & TagsPro1416~Thr1669 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point7.8
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  • Recombinant Collagen Type IV Alpha 1 (COL4a1) Packages (Simulation)
  • Recombinant Collagen Type IV Alpha 1 (COL4a1) Packages (Simulation)
  • RPA149Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Peritoneal Dissemination Requires an Sp1-Dependent CXCR4/CXCL12 Signaling Axis and Extracellular Matrix–Directed Spheroid FormationPubmed:26744523
  • miR-146a regulates glucose induced upregulation of inflammatory cytokines extracellular matrix proteins in the retina and kidney in diabetes10.1371
  • Endothelin-1 traps potently reduce pathologic markers back to basal levels in an in vitro model of diabetes
  • Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM diseasePubmed: 30817909
  • In vivo studies demonstrate that endothelin-1 traps are a potential therapy for type I diabetes
  • Curcumin Analogs Reduce Stress and Inflammation Indices in Experimental Models of DiabetesPubmed: 31920992

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