Recombinant Cytochrome P450 7A1 (CYP7A1) Mus musculus (Mouse) Recombinant protein

CYP7; CYP7-A1; CYPVII; Cholesterol 7 Alpha Hydroxylase; Cytochrome P450 Family 7 Subfamily A Polypeptide 1

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationEndoplasmic reticulum membrane; Peripheral membrane protein. Microsome membra
  • Molecular Mass 31.5kDa, Accurate 31.34kDa(Analysis of differences refer to the manual)
  • Residues & TagsLeu227~Leu467 (Accession # Q64505) with
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point6.9
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  • Recombinant Cytochrome P450 7A1 (CYP7A1) Packages (Simulation)
  • Recombinant Cytochrome P450 7A1 (CYP7A1) Packages (Simulation)
  • RPB053Mu01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in PBS or others.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Parabacteroides distasonis Alleviates Obesity and Metabolic Dysfunctions via Production of Succinate and Secondary Bile AcidsPubmed: 30605678
  • Hyperglycemia exacerbates dyslipidemia-induced changes in uptake, synthesis, and transporters of bile acids in rats: Assessment of restorative potentials of ALA and?…Doi: 10.1016/j.jff.2019.01.038
  • Withaferin-A down-regulate enterohepatic circulation of bile acids: An insight from a hyperlipidemic rat model

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