Recombinant Deoxyribonuclease I (DNASE1)

DNaseI; DNL1; DRNI; DNase-I; Dornase alfa

UOM
1. 10µg
2. 50µg
3. 200µg
4. 1mg
5. 5mg
FOB US$ 112.00 US$ 280.00 US$ 560.00 US$ 1,680.00 US$ 4,200.00
Quantity

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Overview

Properties

Product No.RPB127Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
Applications Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase Developmental science

Source Prokaryotic expression, Host E.coli
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
Subcellular LocationNucleus, Secreted
Molecular Mass 28.3kDa, Accurate 28kDa(Analysis of differences refer to the manual)
Residues & TagsGly19~Ala259 with N-terminal His Tag
Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Traits Freeze-dried powder, Purity > 90%
Isoelectric Point4.6

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Packages (Simulation)
Packages (Simulation)

Figure . Gene Sequencing (extract)

Figure. SDS-PAGE

ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service

Increment services

Citations

Low Circulating Levels of Mitochondrial and High Levels of Nuclear DNA Predict Mortality in Chronic Heart FailurePubmed:27349571
Inhibition of nuclease activity by a splice-switching oligonucleotide targeting deoxyribonuclease 1 mRNA prevents apoptosis progression and prolong viability of …Pubmed: 32315661
Biomarkers of neutrophil extracellular traps (NETs) and nitric oxide-(NO)-dependent oxidative stress in women who miscarriedPubmed: 32753622
DNase 1 Protects From Increased Thrombin Generation and Venous Thrombosis During Aging: Cross‐Sectional Study in Mice and HumansPubmed:35023342