Recombinant Enolase 1 (ENO1) Bos taurus; Bovine (Cattle) Recombinant protein

NNE; ENO1L1; MPB1; PPH; Alpha Enolase; Enolase 1; Phosphopyruvate hydratase; Plasminogen-binding protein; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Cytoplasm
  • Molecular Mass 48.9kDa, Accurate 48kDa(Analysis of differences refer to the manual)
  • Residues & TagsMet1~Lys434 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point6.5
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  • Recombinant Enolase 1 (ENO1) Packages (Simulation)
  • Recombinant Enolase 1 (ENO1) Packages (Simulation)
  • RPB449Bo01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensorPubMed: 20557064
  • Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodulesPubmed:27172385
  • Putative salivary biomarkers useful to differentiate patients with fibromyalgiaPubmed:29654921
  • The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of EndometriosisPubmed: 33062681
  • Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins
  • Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression34376212
  • Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinomaPubmed:35610567

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