Recombinant Epithelial Cell Adhesion Molecule (EPCAM)    
    
CD326; TACSTD1; EGP; KSA; M4S1; MIC18; TROP1; HEGP2; ESA; Epithelial glycoprotein; Adenocarcinoma-associated antigen; Tumor Associated Calcium Signal Transducer 1
Overview
Properties
- Product No.RPB283Hu01
 - Organism SpeciesHomo sapiens (Human) Same name, Different species.
 - 
                                Applications
                                Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only - DownloadInstruction Manual
 - CategoryCD & Adhesion molecule
 
- Source Prokaryotic expression, Host E.coli
 - Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
 - Subcellular LocationMembrane
 - Molecular Mass 57.3kDa, Accurate 62kDa(Analysis of differences refer to the manual)
 - Residues & TagsGln24~Leu264 with N-terminal His and GST Tag
 - Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
 - Traits Freeze-dried powder, Purity > 95%
 - Isoelectric Point5.6
 
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            Sequence
                                        
                                    Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
- 
                
                    Endotoxin Removal Kit
                                 - 
                
                    BCA Protein Quantification Kit
                                 - 
                
                    Protein Labeling Customized Service
                                 - 
                
                    Molecular Mass Marker for Protein
                                 - 
                
                    Recombinant Protein Customized Service
                                 - 
                
                    Monoclonal Antibody Customized Service
                                 - 
                
                    Polyclonal Antibody Customized Service
                                 - 
                
                    Protein Activity Test Experiment Service
                                 - 
                
                    Immunoprecipitation (IP) Experiment Service
                                 - 
                
                    Buffer
                                 - 
                
                    Endotoxin Removal Kit II
                                 - 
                
                    Real Time PCR Experimental Service
                                 - 
                
                    Spike RBD Protein (S-RBD)
                                 - 
                
                    Protein G
                                 - 
                
                    Protein A
                                 
Citations
- Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platformScience: Article
 - Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determinationscience:S0026265X16300091
 - Role of several cytokines and adhesion molecules in the diagnosis and prediction of survival ofhepatocellular carcinoma.pubmed:27916547
 - Integrated bio-affinity nano-platform into a microfluidic immunosensor based on monoclonal bispecific trifunctional antibodies for the electrochemical determination of …pubmed:27836687
 - Integrated bio-affinity nano-platform into a microfluidic immunosensor based on monoclonal bispecific trifunctional antibodies for the electrochemical determination of epithelial cancer biomarker10.1016/j.cca.2016.11.012
 
