Eukaryotic Leukemia Inhibitory Factor (LIF) Bos taurus; Bovine (Cattle) Eukaryotic protein

CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin

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Overview
Properties
  • Source Eukaryotic expression, Host 293F Cell
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 20.8kDa, Accurate 40kDa(Analysis of differences refer to the manual)
  • Residues & TagsSer23~Ala196 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point9.3
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  • Eukaryotic Leukemia Inhibitory Factor (LIF) Packages (Simulation)
  • Eukaryotic Leukemia Inhibitory Factor (LIF) Packages (Simulation)
  • EPA085Bo61.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Leukemia inhibitory factor (LIF) potentiates antinociception activity and inhibits tolerance induction of opioidspubmed:28077540
  • A study of altered cytokine rhythms associated with successful implantation in cows10.1080/09291016.2017.1361159
  • Integrated analysis of cancer stem cells-associated lncRNA-miRNA-mRNA network for ovarian cancer via microarray and Gene Expression Omnibus database

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