Eukaryotic Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)
ARMET; ARP; Arginine Rich Mutated In Early Stage Tumors; Arginine-rich protein
Overview
Properties
- Product No.EPC300Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only - DownloadInstruction Manual
- CategoryTumor immunityNeuro science
- Source Eukaryotic expression, Host 293F cell
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationSecreted
- Molecular Mass 19.8kDa, Accurate 21kDa(Analysis of differences refer to the manual)
- Residues & TagsLeu25~Leu182 with N-terminal His Tag
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder, Purity > 97%
- Isoelectric Point8.4
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Sequence

Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
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BCA Protein Quantification Kit
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Protein Labeling Customized Service
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Molecular Mass Marker for Protein
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
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Buffer
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Lentivirus Packaging Experiment Service
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Adenovirus Packaging Experiment Service
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Citations
- Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome SequencingPubMed: PMC3378547
- Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance.pubmed:28216543
- Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signalingpubmed:28263910
- Circulating mesencephalic astrocyte-derived neurotrophic factor is increased in newly diagnosed prediabetic and diabetic patients, and is associated with insulin resistance10.1507:endocrj.EJ16-0472
- MANF regulates metabolic and immune homeostasis in ageing and protects against liver damage
- Feeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway33856409
- The Potential Protective Effect of Mesencephalic Astrocyte-Derived Neurotrophic Factor on Post-Operative Delirium via Inhibiting Inflammation and Microglia …34234505