Eukaryotic Neprilysin (CD10)

NEP; MME; CALLA; SFE; Atriopeptidase; Enkephalinase; Skin fibroblast elastase; Common Acute Lymphoblastic Leukemia Antigen; Membrane Metallo-Endopeptidase

UOM
1. 10µg
2. 50µg
3. 200µg
4. 1mg
5. 5mg
FOB US$ 260.00 US$ 650.00 US$ 1,300.00 US$ 3,900.00 US$ 9,750.00
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Overview

Properties

Product No.EPB785Hu61
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
Applications Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase Neuro science

Source Eukaryotic expression, Host 293F cell
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
Subcellular LocationMembrane
Molecular Mass 81.6kDa, Accurate 100kDa(Analysis of differences refer to the manual)
Residues & TagsTyr52~Trp750 with N-terminal His Tag
Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
Traits Freeze-dried powder, Purity > 95%
Isoelectric Point5.2

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Packages (Simulation)
Packages (Simulation)

Figure . Gene Sequencing (extract)

Figure. SDS-PAGE

ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service

Increment services

Citations

Elevated Plasma levels of Neutral Endpeptidase in Hypertension Patients Treated with Angiotensin Converting Enzyme InhibitorsAhajournals:Source
Effect of High Glucose Levels on Amyloid β Production in Retinas of Spontaneous Diabetes Mellitus Otsuka Long-Evans Tokushima Fatty RatsPubMed: 25832640
Soluble neprilysin does not correlate with outcome in heart failure with preserved ejection fractionPubmed:26725876
A Test in Context: Neprilysin: Function, Inhibition, and Biomarker.pubmed:27491909
Effects of sacubitril/valsartan on neprilysin targets and the metabolism of natriuretic peptides in chronic heart failure: a mechanistic clinical studyPubmed: 30520545
Increased granulocyte membrane neprilysin (CD10) expression is associated with better prognosis in heart failurePubmed: 30828920
Soluble neprilysin, NT-proBNP, and growth differentiation factor-15 as biomarkers for heart failure in dialysis patients (SONGBIRD)Pubmed: 32002632
Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in micePubmed: 32522471
P1620 Association of granulocyte neprilysin (CD10) expression with prognosis in heart failure with reduced ejection fraction
Finding a reliable assay for soluble neprilysinPubmed:35331754
The potential role of serum tau protein (MAPT), neuronal cell adhesion molecule (NrCAM) and neprilysin (NEP) in neurodegenerative disorders development in …