Recombinant Extracellular Signal Regulated Kinase 1 (ERK1) Homo sapiens (Human) Recombinant protein

HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; MAPK3; ERT2; Mitogen-Activated Protein Kinase 3; Insulin-stimulated MAP2 kinase; Microtubule-associated protein 2 kinase

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationNucleus, Cytoplasm
  • Molecular Mass 31.3kDa, Accurate 28kDa(Analysis of differences refer to the manual)
  • Residues & TagsPro75~Thr312 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point8.7
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  • Recombinant Extracellular Signal Regulated Kinase 1 (ERK1) Packages (Simulation)
  • Recombinant Extracellular Signal Regulated Kinase 1 (ERK1) Packages (Simulation)
  • Recombinant Extracellular Signal Regulated Kinase 1 (ERK1) Figure . Gene Sequencing (extract)
  • RPB357Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndromepubmed:27538909
  • Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis33575317
  • Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026

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