Recombinant Glucose 6 Phosphate Isomerase (GPI) 
AMF; NLK; PHI; SA36; Phosphoglucose Isomerase; Autocrine motility factor; Neuroleukin; Phosphohexose isomerase; Sperm antigen 36
Overview
Properties
- Product No.RPA725Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathway
- Source Prokaryotic expression, Host E.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationSecreted, Cytoplasm
- Molecular Mass 28kDa, Accurate 26kDa(Analysis of differences refer to the manual)
- Residues & TagsMet263~Asn475 with N-terminal His Tag
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder, Purity > 90%
- Isoelectric Point7.9
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Packages (Simulation)
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Packages (Simulation)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
Sequence
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
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Endotoxin Removal Kit
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BCA Protein Quantification Kit
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Protein Labeling Customized Service
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Molecular Mass Marker for Protein
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Recombinant Protein Customized Service
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Buffer
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Endotoxin Removal Kit II
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Citations
- Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factorPubMed: 23027359
- Bruton's tyrosine kinase deficiency inhibits autoimmune arthritis but fails to block immune complex‐mediated inflammatory arthritisPubmed:26945549
- Bruton's Tyrosine Kinase Deficiency Inhibits Autoimmune Arthritis in Mice but Fails to Block Immune Complex–Mediated Inflammatory Arthritispubmed:26945549
- The GPI transamidase complex subunit PbGPI16 of Plasmodium berghei is important for inducing experimental cerebral malariaPubmed:29784863
- Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motilityPubmed: 31394311
- The Asthma-associated PER1-like domain-containing protein 1 (PERLD1) Haplotype Influences Soluble Glycosylphosphatidylinositol Anchor Protein (sGPI …Pubmed: 31959860
- T–B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated ProteinPubmed: 33199538