Recombinant Glutamine synthetase (GS) Homo sapiens (Human) Recombinant protein

GLNS; GLUL; Glutamate-Ammonia Ligase; Glutamate decarboxylase

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Mitochondrion, Cytoplasm
  • Molecular Mass 43.6kDa, Accurate 45kDa(Analysis of differences refer to the manual)
  • Residues & TagsMet1~Asn373 (Accession # P15104) with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point6.5
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  • Recombinant Glutamine synthetase (GS) Packages (Simulation)
  • Recombinant Glutamine synthetase (GS) Packages (Simulation)
  • RPD761Hu01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Cargo proteins of plasma astrocyte-derived exosomes in Alzheimer’s diseasepubmed:27511944
  • High complement levels in astrocyte‐derived exosomes of Alzheimer diseasePubmed:29406582
  • Sex differences in the glutamate signaling pathway in juvenile ratsPubmed:28861894
  • Deficient neurotrophic factors of CSPG4-type neural cell exosomes in Alzheimer diseasePubmed:29924942
  • Glutamine synthetase promotes tumor invasion in hepatocellular carcinoma through mediating epithelial–mesenchymal transitionPubmed: 31652385
  • Traumatic brain injury increases plasma astrocyte‐derived exosome levels of neurotoxic complement proteinsPubmed: 31916313
  • Decreased mitochondrial electron transport proteins and increased complement mediators in plasma neural-derived exosomes of early psychosisPubmed: 33106473
  • Drug Repurposing of Asparaginase and Vitamin C Targeting Glutamine Synthetase Improves Anticancer Effect in Metastatic Castration-resistant Prostate …

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