Recombinant Glypican 4 (GPC4) 
K-Glypican; Glypican Proteoglycan 4; Secreted glypican-4
Overview
Properties
- Product No.RPA998Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only - DownloadInstruction Manual
- CategoryTumor immunityDevelopmental science
- Source Prokaryotic expression, Host E.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationMembrane, Extracellular matrix
- Molecular Mass 24.2kDa, Accurate 27kDa(Analysis of differences refer to the manual)
- Residues & TagsThr220~Gly426 with N-terminal His Tag
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder, Purity > 97%
- Isoelectric Point6.5
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Packages (Simulation)
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Packages (Simulation)
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Figure . Gene Sequencing (extract)
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SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
Sequence
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
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Endotoxin Removal Kit
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BCA Protein Quantification Kit
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Protein Labeling Customized Service
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Molecular Mass Marker for Protein
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Recombinant Protein Customized Service
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Buffer
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Endotoxin Removal Kit II
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Citations
- Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammationPubmed:Pmc4072672
- Aspects of fluid therapy in the critically ill. Experimental and clinical studies on fluid therapy in the inflammatory conditions.250690_2_G5_Svajunas_S.pdf
- Elevated plasma glypicans are associated with organ failure in patients with infectionPubmed: 30618011
- Albumin infusion rate and plasma volume expansion: a randomized clinical trial in postoperative patients after major surgeryPubmed: 31138247
- Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with …Pubmed: 32816247
- Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study
- Glypican-4 in pregnancy and its relation to glucose metabolism, insulin resistance and gestational diabetes mellitus status34903856