Recombinant Heparan Sulfate Proteoglycan (HSPG) Homo sapiens (Human) Recombinant protein

SDC2; HSPG1; SYND2; Fibroglycan; Syndecan 2; Syndecan Proteoglycan 2; Heparan Sulfate Proteoglycan 1,Cell Surface-Associated; Heparan Sulphate Proteoglycan

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Molecular Mass 15.2kDa, Accurate 23kDa(Analysis of differences refer to the manual)
  • Residues & TagsGlu19~Glu144 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point4.7
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  • Recombinant Heparan Sulfate Proteoglycan (HSPG) Packages (Simulation)
  • Recombinant Heparan Sulfate Proteoglycan (HSPG) Packages (Simulation)
  • Recombinant Heparan Sulfate Proteoglycan (HSPG) Figure . Gene Sequencing (extract)
  • RPA565Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Cytotoxic effects of antiglypican-3 against HepG3 cell lineEbscohost: Source
  • Fibrinogen, an endogenous ligand of Toll-like receptor 4, activates monocytes in pre-eclamptic patientsPubmed: 24661950
  • Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzymePubmed: 24530413
  • Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinomaPubmed:25449037
  • Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic RelevancePubmed:25692257
  • Endothelial glycocalyx layer shedding following lung resectionpubmed:27643669
  • Circulating syndecans during critical illness.pubmed:28256016
  • Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 andPubmed:29669302
  • Glioma targeting peptide modified apoferritin nanocagePubmed:29726297
  • Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypassPubmed: 30687934
  • Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period …
  • Heparin prevents in vitro glycocalyx shedding induced by plasma from COVID-19 patients33781826
  • A novel mutation in ext2 caused hereditary multiple exostoses through reducing the synthesis of heparan sulfate34042151
  • Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine?¡­33518110
  • The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo …34423832

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