Recombinant Hexosaminidase B Beta (HEXb) Rattus norvegicus (Rat) Recombinant protein

Hex-B; ENC-1AS; HCC-7; Beta-N-acetylhexosaminidase subunit beta; Cervical cancer proto-oncogene 7 protein; N-acetyl-beta-glucosaminidase subunit beta

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationLysosome
  • Molecular Mass 17.1kDa, Accurate 17kDa(Analysis of differences refer to the manual)
  • Residues & TagsPro100~Ile216 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point5.4
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  • Recombinant Hexosaminidase B Beta (HEXb) Packages (Simulation)
  • Recombinant Hexosaminidase B Beta (HEXb) Packages (Simulation)
  • RPA637Ra01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Uncovering the Molecular Mechanism of Anti-Allergic Activity of Silkworm Pupa-Grown Cordyceps militaris Fruit Body.pubmed:28367714
  • Resveratrol efficiently improves pulmonary function via stabilizing mast cells in a rat intestinal injury modelpubmed:28733146
  • Functionality of a bicistronic construction containing HEXA and HEXB genes encoding ¦Â-hexosaminidase A for cell-mediated therapy of GM2 gangliosidoses34100447
  • The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets34099640

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