Recombinant High Molecular Weight Kininogen (HMWK)
Williams-Fitzgerald-Flaujeac Factor; Fitzgerald Factor; HMWK-Kallikrein Factor
Overview
Properties
- Product No.RPB805Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & KinaseHematology
- Source Prokaryotic expression, Host E.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationSecreted, Extracellular matrix
- Molecular Mass 60.6kDa, Accurate 53kDa(Analysis of differences refer to the manual)
- Residues & TagsArg268~Arg540 with Two N-terminal Tags, His-tag and SUMO-tag
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder, Purity > 80%
- Isoelectric Point8.3
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Sequence

Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
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BCA Protein Quantification Kit
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Molecular Mass Marker for Protein
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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TBST Buffer
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TBS Buffer
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TRIS Buffer
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Citations
- Factor XII–mediated contact activation related to poor prognosis in disseminated intravascular coagulationPubMed: 26706311
- Factor XII-mediated contact activation related to poor prognosis in disseminated intravascular coaCavia (Guinea pig )lationPubmed:26706311
- Contact system activation and high thrombin generation in hyperthyroidism.pubmed:28137735
- Contact system activation in disseminated intravascular coagulation: activities of prekallikrein and high-molecular-weight kininogen are significant risk factorsPubmed:34993714