Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a)
HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
Overview
Properties
- Product No.RPA798Bo02
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only - DownloadInstruction Manual
- CategoryTumor immunityCardiovascular biologyReproductive science
- Source Prokaryotic expression, Host E.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationNucleus, Cytoplasm
- Molecular Mass 65.02kDa, Accurate 65kDa(Analysis of differences refer to the manual)
- Residues & TagsLeu220~Asp349 (Accession # Q9XTA5) with Two N-terminal Tags, His-tag and MBP-tag
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder, Purity > 90%
- Isoelectric Point6.13
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Sequence

Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
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Endotoxin Removal Kit
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BCA Protein Quantification Kit
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Protein Labeling Customized Service
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Molecular Mass Marker for Protein
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Recombinant Protein Customized Service
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Buffer
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Endotoxin Removal Kit II
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Citations
- Quercetin greatly improved therapeutic index of doxorubicin against 4T1 breast cancer by its opposing effects on HIF-1α in tumor and normal cells.PubMed: 19466611
- Baicalin suppresses lung carcinoma and lung metastasis by SOD mimic and HIF-1α inhibitionScienceDirect: S0014299909011352
- Lactate Dehydrogenase 5 Expression in Non-Hodgkin Lymphoma Is Associated with the Induced Hypoxia Regulated Protein and Poor PrognosisPubMed: PMC3781153
- Selective inhibition of PKCβ2 preserves cardiac function after myocardial infarction and is associated with improved angiogenesis of ischemic myocardium in diabetic ratsPubmed: 24002353
- Modulation of arginine and asymmetric dimethylarginine concentrations in liver and plasma by exogenous hydrogen sulfide in LPS-induced endotoxemiaPubmed: 24289078
- Method for preventing or treating a disease, disorder or condition induced by retina ischemiaUS8569372
- Effects of adrenomedullin and vascular endothelial growth factor on ischemia/reperfusion injury in skeletal muscle in rats.Pubmed: 23816242
- HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa KeratitisPlos: Source
- Telomeres are elongated in rats exposed to moderate altitudePubmed:Pmc4088304
- Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1α protein.Pubmed:25384583
- The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1α and VEGFPubmed:25445537
- Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic ratsPubmed:25524396
- Maternal omega-3 fatty acid supplementation to a vitamin B 12 deficient diet normalizes angiogenic markers in the pup brain at birthPubMed: 25889224
- Determination of the Preferred Conditions for the Isolated Perfusion of Porcine KidneysPubMed: 25341590
- Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic systemPubMed: 25905434
- Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariaepmc:PMC4794592
- Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1αPubmed:27121973
- Использование гипоксического прекондиционирования для подготовки аутодермотрансплантата у больных со скомпрометированной микроциркуляцией91
- Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α.pubmed:27121973
- Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritisetm:13
- Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trialissn:2542-3975
- R132H Mutation in IDH1 Gene is Associated with Increased Tumor HIF1-Alpha and Serum VEGF Levels in Primary Glioblastoma Multiforme pubmed:28667042
- Is there any relationship between adipocytokines and angiogenesis factors to address endothelial dysfunction and platelet aggregation in untreated patients with preeclampsia?10.1007/s00404-017-4461-2
- Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in kneepubmed:28123469
- Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation10.1016/j.mjafi.2017.04.003
- Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxiaPubmed:29454386
- Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate HypoxiaPubmed:29308565
- Determination of hypoxia-inducible factor-1 by using a ratiometric colorimetric test based on click-mediated growth of gold nanoparticlesPubmed: 30209641
- Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in …Pubmed: 30353332
- OKN-007 Increases temozolomide (TMZ) Sensitivity and Suppresses TMZ-Resistant Glioblastoma (GBM) Tumor GrowthPubmed: 30468988
- Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.
- Age-Specific Features of Hypoxia Tolerance and Intensity of Lipopolysaccharide-Induced Systemic Inflammatory Response in Wistar RatsPubmed: 30903510
- The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency
- Vitexin alleviates interleukin‐1β induced inflammatory responses in chondrocytes from osteoarthritis patients: involvement of HIF‐1α pathwayPubmed: 31055848
- A disintegrin‐like and metalloproteinase with thrombospondin‐1 (ADAMTS‐1) levels in gingival crevicular fluid correlate with vascular endothelial growth factor‐A …Pubmed: 31020669
- Hypoxia Preconditioned Adipose Derived Endothelial Progenitor Cells Promote Bladder AugmentationPubmed: 31238789
- ПОТЕНЦИРОВАНИЕ АНТИГИПОКСАНТАМИ ЭФФЕКТА ГИПОКСИЧЕСКОГО ПРЕКОНДИЦИОНИРОВАНИЯ
- Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsiaPubmed: 31420792
- Regulation of MicroRNA-155 and Its Related Genes Expression by Inositol Hexaphosphate in Colon Cancer CellsPubmed: 31744065
- Short-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callusPubmed: 32894200
- PLCε regulates metabolism and metastasis signaling via HIF‐1α/MEK/ERK pathway in prostate cancerPubmed: 32383180
- Гипоксия-зависимый транскрипционный контроль активности деструктивных изменений пародонта воспалительного и злокачественного генеза
- Prenatal vitamin D supplementation reduces blood pressure and improves placental angiogenesis in an animal model of preeclampsia33237074
- Lactucaxanthin protects retinal pigment epithelium from hyperglycemia-regulated hypoxia/ER stress/VEGF pathway mediated angiogenesis in ARPE-19 cell and rat ¡33705802
- Astaxanthin mediated regulation of VEGF through HIF1¦Á and XBP1 signaling pathway: An insight from ARPE-19 cell and streptozotocin mediated diabetic rat model33789142
- Serum hypoxia-inducible factor-1¦Á and uterine artery Doppler ultrasound during the first trimester for prediction of preeclampsia33758274
- Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377