Recombinant Interleukin 12A (IL12A) Canis familiaris; Canine (Dog) Recombinant protein

p35; IL12-A; CLMF1; NFSK; NKSF1; Natural Killer Cell Stimulatory Factor 1; Cytotoxic Lymphocyte Maturation Factor 1; NF Cell Stimulatory Factor Chain 1

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 20.1kDa, Accurate 25kDa(Analysis of differences refer to the manual)
  • Residues & TagsLeu41~Leu186 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point5
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  • Recombinant Interleukin 12A (IL12A) Packages (Simulation)
  • Recombinant Interleukin 12A (IL12A) Packages (Simulation)
  • RPA059Ca01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Evaluation of serum concentrations of interleukin (IL)-4, IL-10, and IL-12 during pregnancy in bitchesScienceDirect: S0093691X1300037X
  • Establishment and evaluation of a bead-based luminex assay allowing simultaneous quantification of equine IL-12 and IFN-γ.Pubmed: 23564769
  • Impact of purified oat 1-3,1-4-b-d-glucan of different molecular weight on alleviation of inflammation parameters during gastritis:
  • Impact of purified oat 1-3, 1-4-β-d-glucan of different molecular weight on alleviation of inflammation parameters during gastritisS1756464616303528
  • Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies33999928

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