Recombinant Mucin 2 (MUC2) Rattus norvegicus (Rat) Recombinant protein

MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 66.7kDa, Accurate 70kDa(Analysis of differences refer to the manual)
  • Residues & TagsVal33~Ala601 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point4.9
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  • Recombinant Mucin 2 (MUC2) Packages (Simulation)
  • Recombinant Mucin 2 (MUC2) Packages (Simulation)
  • Recombinant Mucin 2 (MUC2) Figure . Gene Sequencing (extract)
  • RPA705Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.PubMed: 20717734
  • Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats.Pubmed:24627600
  • Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosisPubMed: 26217323
  • Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathwayPubMed: 26244071
  • Secreted mucins and airway bacterial colonization in non-CF bronchiectasisPubMed: 26172851
  • Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonizationdoi:10.1513
  • An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489
  • Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA10.21769:BioProtoc.2879
  • The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung diseasePubmed: 30573494
  • Clinical Significance of Serum Membrane-Bound Mucin-2 Levels in Breast CancerPubmed: 30682816
  • PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific MetabolismPubmed: 31564549
  • Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic RatsPubmed: 32144600
  • Mucus layer modeling of human colonoids during infection with enteroaggragative E. coliPubmed: 32601325
  • Defective intestinal repair after short-term high fat diet due to loss of efferocytosis
  • Inhibition of cyclooxygenase©\2 enhanced intestinal epithelial homeostasis via suppressing ¦Â©\catenin signalling pathway in experimental liver fibrosis34145945
  • Reduced acetylcholine and elevated muscarinic receptor 2 in duodenal mucosa contribute to the impairment of mucus secretion in 6-hydroxydopamine-induced …34370080

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