Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1) Rattus norvegicus (Rat) Recombinant protein

NOS; nNOS; IHPS1; NC-NOS; N-NOS; bNOS; Neuronal NOS; Constitutive NOS; NOS type I; Nitric Ocide Synthase; Peptidyl-cysteine S-nitrosylase NOS1

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Molecular Mass 20.2kDa, Accurate 16kDa(Analysis of differences refer to the manual)
  • Residues & TagsArg726~Asp894 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point9.0
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  • Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1) Packages (Simulation)
  • Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1) Packages (Simulation)
  • Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1) Figure. Gene Sequencing (Extract)
  • RPA815Ra03.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric OxidePubMed: 23359814
  • The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disordersPubmed:25245337
  • Состояние эндотелиально-сосудистого фактора у беременных с артериальной гипертензиейhandle:123
  • Clinical antibacterial effectiveness and biocompatibility of gaseous ozone after incomplete caries removalPubmed:29858659
  • Antioxydant and Endotheliotropic Properties 4-Hydroxy-3, 5-Di-Tret-Butyl Cinnamic Acid under Conditions of Experimental Brain Ischemia:
  • Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in RatsDoi: 10.5152/eurasianjmed.2018.18160
  • The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT CellsPubmed: 32104540
  • Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial …Pubmed: 31734543
  • Protective Effects of Rocuronium Bromide on Ischemia-Reperfusion Injury in Skeletal Muscle Induced by Tourniquet in Patients Undergoing Elective Unilateral Total …Pubmed: 32884241
  • Evaluation of salivary and serum methylated arginine metabolites and nitric oxide synthase in advanced periodontitis patientsPubmed:35426000

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