Recombinant Octamer Binding Transcription Factor 4 (OCT4)     
     
POU5F1; OCT3; OTF3; OTF4; Octamer-4; POU Domain Class 5 Transcription Factor 1; POU Class 5 Homoeobox 1; Octamer-binding protein 3
Overview
Properties
- Product No.RPA424Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- 
                                Applications
                                Positive Control; Immunogen; SDS-PAGE; WB.
 If bio-activity of the protein is needed, please check active protein.
 Research use only
- DownloadInstruction Manual
- CategorySignal transductionDevelopmental science
- Source Prokaryotic expression, Host E.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationSecreted
- Molecular Mass 29.2kDa, Accurate 29kDa(Analysis of differences refer to the manual)
- Residues & TagsSer129~Asn352 with N-terminal His Tag
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder, Purity > 97%
- Isoelectric Point9.4
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            Sequence
 
                                        
                                    Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
- 
                 Endotoxin Removal Kit Endotoxin Removal Kit
- 
                 BCA Protein Quantification Kit BCA Protein Quantification Kit
- 
                 Protein Labeling Customized Service Protein Labeling Customized Service
- 
                 Molecular Mass Marker for Protein Molecular Mass Marker for Protein
- 
                 Recombinant Protein Customized Service Recombinant Protein Customized Service
- 
                 Monoclonal Antibody Customized Service Monoclonal Antibody Customized Service
- 
                 Polyclonal Antibody Customized Service Polyclonal Antibody Customized Service
- 
                 Protein Activity Test Experiment Service Protein Activity Test Experiment Service
- 
                 Immunoprecipitation (IP) Experiment Service Immunoprecipitation (IP) Experiment Service
- 
                 Buffer Buffer
- 
                 Endotoxin Removal Kit II Endotoxin Removal Kit II
- 
                 Real Time PCR Experimental Service Real Time PCR Experimental Service
- 
                 Spike RBD Protein (S-RBD) Spike RBD Protein (S-RBD)
- 
                 Protein G Protein G
- 
                 Protein A Protein A
Citations
- A universal glucometer-based biosensor for portable and quantitative detection of transcription factorsRsc: Source
- Upregulation of stem cell markers ALDH1A1 and OCT4 as potential biomarkers for the early detection of cervical carcinomaDoi: 10.3892/ol.2018.9381
- Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+ T cells of healthy individuals of Indian origin
- Experimental and Modeling Framework for the Production of Cell and Protein-Based Biopharmaceutical Products
- Generation and characterization of three clones (NCCSi007A, NCCSi007B and NCCSi007C) of an integration free induced Pluripotent Stem Cell line from a …Pubmed: 31862609
- Studies on male gonadal toxicity of bisphenol A in diabetic rats: An example of exacerbation effectPubmed:35187753

 
         
    
     
                 
                 
                             
                