Recombinant Peroxisome Proliferator Activated Receptor Gamma (PPARg) Rattus norvegicus (Rat) Recombinant protein

PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationNucleus, Cytoplasm
  • Molecular Mass 22.3kDa, Accurate 22kDa(Analysis of differences refer to the manual)
  • Residues & TagsGln311~Leu493 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point5.5
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  • Recombinant Peroxisome Proliferator Activated Receptor Gamma (PPARg) Packages (Simulation)
  • Recombinant Peroxisome Proliferator Activated Receptor Gamma (PPARg) Packages (Simulation)
  • Recombinant Peroxisome Proliferator Activated Receptor Gamma (PPARg) Figure. Gene Sequencing (Extract)
  • RPA886Ra01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Elevated levels of PPAR-gamma in the cerebrospinal fluid of patients with multiple sclerosisPubmed: 24021801
  • Nonivamide enhances miRNA let‐7d expression and decreases adipogenesis PPARγ expression in 3T3‐L1 cellsPubmed:25704235
  • Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazonePubMed: 26321377
  • Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic Rats Supplemented with Momordica charantiapmc:PMC4866090
  • Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic RatsSupplemented with Momordica charantia.pubmed:27190792
  • Establishment of a rabbit model to study the influence of advanced glycation end productsaccumulation on osteoarthritis and the protective effect of pioglitazone.pubmed:26321377
  • Unlike PPARgamma, neither other PPARs nor PGC-1alpha is elevated in the cerebrospinal fluid of patients with multiple sclerosispubmed:28483651
  • Engulfment of Hb‐activated platelets differentiates monocytes into pro‐inflammatory macrophages in PNH patientsPubmed:29677388
  • Simpson–Golabi–Behmel syndrome human adipocytes reveal a changing phenotype throughout differentiationPubmed:29574488
  • Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsiaPubmed: 31420792
  • Hyperglycemia Changes Expression of Key Adipogenesis Markers (C/EBPα and PPARᵞ) and Morphology of Differentiating Human Visceral AdipocytesPubmed: 31398873
  • Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cellsPubmed: 31916655
  • In©\vitro effect of pine bark extract on melanin synthesis, tyrosinase activity, production of endothelin©\1 and PPAR in cultured melanocytes exposed to Ultraviolet?¡­33960120
  • TRPA1 Agonist Cinnamaldehyde Decreases Adipogenesis in 3T3-L1 Cells More Potently than the Non-agonist Structural Analog Cinnamyl Isobutyrate33403292
  • Quantitative real-time measurement of endothelin-1-induced contraction in single non-activated hepatic stellate cells34343209
  • Maternal Vitamin D Deficiency Reduces Docosahexaenoic Acid, Placental Growth Factor and Peroxisome Proliferator Activated Receptor Gamma levels in the Pup …34768025
  • Melatonin attenuates cisplatin-induced acute kidney injury in mice: Involvement of PPARα and fatty acid oxidationPubmed:35367536

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