Recombinant Platelet Derived Growth Factor Subunit B (PDGFB) Sus scrofa; Porcine (Pig) Recombinant protein

PDGF-B; PDGF2; SIS; SSV; C-sis; Simian Sarcoma Viral(v-sis)Oncogene Homolog; Platelet Derived Growth Factor Beta Polypeptide; Proto-oncogene c-Sis; Becaplermin

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Extracellular matrix
  • Molecular Mass 21kDa, Accurate 26kDa(Analysis of differences refer to the manual)
  • Residues & TagsRAGCLSLRVGVGPSASAALDGPPLPTLTEFPLPP+Gly84~Arg199+GNHSSRVC with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point9.4
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  • Recombinant Platelet Derived Growth Factor Subunit B (PDGFB) Packages (Simulation)
  • Recombinant Platelet Derived Growth Factor Subunit B (PDGFB) Packages (Simulation)
  • RPC921Po01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Inhibition of Glomerular Mesangial Cell Proliferation by siPDGF-B-and siPDGFR-β-Containing Chitosan Nanoplexespubmed:27975193
  • In vitro PDGF-B gene silencing studies and In vivo delivery of siRNA to the rat kidney using Chitosan/siRNA nanoplexes5000184338
  • Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagensISSN:1936-2625/IJCEP0058424
  • Melatonin promotes osteoblastic differentiation and regulates PDGF/AKT signaling pathwayPubmed: 31535749
  • PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosisPubmed: 32208584
  • Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders33868627

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