Recombinant Apolipoprotein B100 (APOB100)
Apo-B100
Overview
Properties
- Product No.RPA603Bo01
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
-
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayEndocrinologyCardiovascular biologyHepatologyNutrition metabolism
- Source Prokaryotic expression, Host E.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationCytoplasm
- Molecular Mass 31.4kDa, Accurate 30kDa(Analysis of differences refer to the manual)
- Residues & TagsThr4331~Phe4567 with N-terminal His Tag
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder, Purity > 90%
- Isoelectric Point5.0
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Sequence
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
- BCA Protein Quantification Kit
- Molecular Mass Marker for Protein
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Immunoprecipitation (IP) Experiment Service
- TBST Buffer
- TBS Buffer
- TRIS Buffer
- Spike RBD Protein (S-RBD)
- Protein G
- Protein A
Citations
- Enhancement of Naringenin Bioavailability by Complexation with Hydroxypropoyl-β-CyclodextrinPlosone: 0018033
- Chemical sympathectomy induces arterial accumulation of native and oxidized LDL in hypercholesterolemic ratsScienceDirect: S1566070211004152
- iTRAQ-based proteomic profiling of human serum reveals down-regulation of platelet basic protein and apolipoprotein B100 in patients with hematotoxicity induced by chronic occupational benzene exposureScienceDirect: S0300483X11004628
- Up-reCavia (Guinea pig )lation of Hnf1α gene expression in the liver of rats with experimentally induced chronic renal failure – A possible link between circulating PCSK9 and triacylglycerol concentrations Pubmed:26978583
- Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patientsPubmed:26680509
- Alleviating VLDL overproduction is an important mechanism for Laminaria japonica polysaccharide to inhibit atherosclerosis in LDLr-/- mice with diet-induced insulin resistance.pubmed:27928899
- Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients.pubmed:26680509
- Choline and betaine ameliorate liver lipid accumulation induced by vitamin B6 deficiency in rats.pubmed:27696964
- Accumulation of Liver Lipids Induced by Vitamin B6 Deficiency Was Effectively Ameliorated by Choline and, to a Lesser Extent, BetainePubmed: 30814419
- Zygophyllum album saponins prevent atherogenic effect induced by deltamethrin via attenuating arterial accumulation of native and oxidized LDL in ratsPubmed: 32105945
- Permethrin induced arterial retention of native and oxidized LDL in rats by promoting inflammation, oxidative stress and affecting LDL receptors, and collagen …Pubmed: 32911180
- GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity34853313
- Postprandial dyslipidemia after a standardized high-fat meal in BMI-matched healthy individuals, and in subjects with prediabetes or Type 2 diabetes34656950
- Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy