Recombinant Creatine Kinase MB Isoenzyme (CKMB) Mus musculus (Mouse) Recombinant protein

CK2; CK-MB; Creatine Kinase,Muscle/Brain

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationCytoplasm
  • Molecular Mass 76kDa, Accurate 73kDa(Analysis of differences refer to the manual)
  • Residues & TagsMet1~Lys381 with N-terminal His and GST Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point7
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  • Recombinant Creatine Kinase MB Isoenzyme (CKMB) Packages (Simulation)
  • Recombinant Creatine Kinase MB Isoenzyme (CKMB) Packages (Simulation)
  • RPA479Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia–Reperfusion InjuryPubMed: 22814936
  • N-Acetylcysteine and Allopurinol Confer Synergy in Attenuating Myocardial Ischemia Injury via Restoring HIF-1α/HO-1 Signaling in Diabetic RatsPubMed: PMC3715528
  • Effects of morphine and sufentanil preconditioning against myocardial ischemic-reperfusion injury in rabbitsPubMed: 26629064
  • Diabetes blocks the cardioprotective effects of sevoflurane postconditioning by impairing Nrf2/Brg1/HO-1 signalingPubmed:26973173
  • Effect of levocarnitine on TIMP-1, ICAM-1 expression of rats with coronary heart disease and its myocardial protection effectPubmed:26972400
  • Circulating miR-126 and miR-499 reflect progression of cardiovascular disease; correlations with uric acid and ejection fractionpubmed:27924211
  • Peroxisome proliferator-activated receptor γ (PPARγ) mediates the protective effect of quercetin against myocardial ischemia-reperfusion injury via …PMC5209473
  • In Vivo Cardioprotective Effects and Pharmacokinetic Profile of N-Propyl Caffeamide AgainstIschemia Reperfusion Injury.pubmed:27480649
  • Cardiac biomarkers in dogs with visceral leishmaniasisS0301-732X2016000300004
  • Remote Limb Ischaemic Postconditioning Protects Against Myocardial Ischaemia/ Reperfusion Injury in Mice: Activation of JAK/STAT3-Mediated Nrf2-Antioxidant Signalling10.1159:481755
  • Morinda Citrifolia (Noni) and Low Dose Aspirin Prevent Apoptotic Cell Death and Oxidative Stress on Isoproterenol Induced Myocardial Infarction in RatsDOI: 10.5152/etd.2017.1732
  • Metformin attenuated endotoxin-induced acute myocarditis via activating AMPKpubmed:28410530
  • Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity andPubmed:29727991
  • GYY4137 protects against myocardial ischemia/reperfusion injury via activation of the PHLPP-1/Akt/Nrf2 signaling pathway in diabetic micePubmed:29605032
  • Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in ratsPubmed:29956781
  • Cyclic helix B peptide ameliorates acute myocardial infarction in mice by inhibiting apoptosis and inflammatory responses
  • Adiponectin Facilitates Postconditioning Cardioprotection through Both AMPK-Dependent Nuclear and AMPK-Independent Mitochondrial STAT3 ActivationPubmed: 32190173
  • Tissue suction-mediated gene transfer to the beating heart in micePubmed: 32027678
  • Deletion of Rap1 protects against myocardial ischemia/reperfusion injury through suppressing cell apoptosis via activation of STAT3 signalingPubmed: 32020680
  • Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injuryPubmed: 32705277
  • Hydrogen sulphide and nitric oxide cooperate in cardioprotection against ischemia/reperfusion injury in isolated rat heartPubmed: 32871779
  • Melatonin Attenuates Anoxia/Reoxygenation Injury by Inhibiting Excessive Mitophagy Through the MT2/SIRT3/FoxO3a Signaling Pathway in H9c2 CellsPubmed: 32546969
  • Biological Safety and Biodistribution of Chitosan NanoparticlesPubmed: 32340313
  • The mRNA expression of Il6 and Pdcd1 are predictive and protective factors for doxorubicin?induced cardiotoxicity33300057
  • Inactivation of TOPK Caused by Hyperglycemia Blocks Diabetic Heart Sensitivity to Sevoflurane Postconditioning by Impairing the PTEN/PI3K/Akt Signaling33986917
  • Long non-coding RNA MALAT1 modulates myocardial ischemia-reperfusion injury though the PI3K/Akt/eNOS pathway by sponging miRNA-133a-3p to target IGF1R …Pubmed:34968461
  • Hyperglycemia-Induced Overexpression of PH Domain Leucine-Rich Repeat Protein Phosphatase 1 (PHLPP1) Compromises the Cardioprotective Effect of Ischemic …Pubmed:35715527
  • Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733Pubmed:35215383
  • Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrestPubmed:35495584
  • MicroRNA-503 Exacerbates Myocardial Ischemia/Reperfusion Injury via Inhibiting PI3K/Akt-and STAT3-Dependent Prosurvival Signaling PathwaysPubmed:35620576
  • Vitamin E Attenuates Cardiomyopathy Via Alleviation of Autophagic Stress

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