Recombinant Interleukin 35 (IL35)
Overview
Properties
- Product No.RPC008Mu02
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only - DownloadInstruction Manual
- CategoryCytokineTumor immunityInfection immunity
- Source Prokaryotic expression, Host E.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationSecreted
- Molecular Mass 49.7kDa, Accurate 50kDa(Analysis of differences refer to the manual)
- Residues & TagsArg23~Ala215+GGGGS*3+Tyr19~Pro228 with N-terminal His Tag
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder, Purity > 90%
- Isoelectric Point9.2
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Sequence

Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
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BCA Protein Quantification Kit
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Molecular Mass Marker for Protein
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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TBST Buffer
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TBS Buffer
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TRIS Buffer
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Citations
- Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia.PubMed: 22783403
- Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of diseasePubmed: 24175856
- What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility?Pubmed: 24368037
- The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemiaScienceDirect: S0198885913005776
- Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic MothersPubmed:24785110
- Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosisPubmed:25323532
- The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancyPubmed:25611266
- Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor ImmunityPubMed: 25962106
- Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid ArthritisPubMed: 26204444
- Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early diseasePubMed: 26231346
- Anti-inflammatory effects of interleukin-35 in acquired aplastic anemiaPubMed: 26282938
- Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 PathwayPubMed: 26085094
- Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopeniaPubMed: 25640666
- New insights into immune mechanisms underlying response to Rituximab in patients with membranous nephropathy: A prospective study and a review of the literaturePubmed:26876383
- Increased plasma concentrations of interleukin 35 in patients with coronary artery diseaseJournal:-19
- The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositispubmed:27502600
- Enhanced LPS-induced activation of IL-27 signalling in sarcoidosispubmed:27492538
- Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory andanti-inflammatory cytokines.pubmed:27460435
- IL-35在牙周炎及口腔扁平苔藓中的表达及相关性分析QK:98604X
- Upregulation of Interleukin 35 in Patients With Endometriosis Stimulates Cell Proliferationpubmed:28659009
- IL‑35 may maintain homeostasis of the immune microenvironment in periodontitisissn:2542-3975
- Synoviocytes-derived Interleukin 35 Potentiates B Cell Response in Patients with Osteoarthritis and Rheumatoid Arthritispubmed:29247146
- Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitropubmed:28713164
- Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia.pubmed:29257310
- Evaluation of serum interleukin- 35 level in children with persistent asthma.pubmed:27996283
- Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratiopubmed:29236782
- Significant association between FOXP3 gene polymorphism and steroid-resistant acute rejection in living donor liver transplantation10.1002/hep4.1052
- Clinical significance of IL-35 expression in the progression of clear cell renal cell carcinoma ISSN:1940-5901
- Interleukin-35 as a predictor of prostate cancer in patients undergoing initial prostate biopsy.pubmed:28761357
- AB0734 Serum il-35 levels in systemic sclerosis and relationship with clinical features10.1136:annrheumdis-2018-eular.2605
- Interleukin-35 as a New Biomarker of Renal Involvement in Lupus Nephritis PatientsPubmed:29576585
- The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertilityPubmed:29914245
- Pro-tumoral immune cell alterations in wild type and Shb-deficient mice in response to 4T1 breast carcinomasPubmed:29721156
- Serum Interleukin 35 Levels in Systemic Sclerosis and Relationship With Clinical Features.Pubmed: 30431486
- Interleukin‑35 is associated with the tumorigenesis and progression of prostate cancer
- Plasmacytoid dendritic cells protect against immune-mediated acute liver injury via IL-35Pubmed: 31264967
- CD4+ FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially ImpairedPubmed: 32111105
- Interleukin-35 in idiopathic inflammatory myopathiesPubmed: 33128920
- IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1, and IGFBP1Pubmed: 33064893
- Interleukin-35 promotes progression of prostate cancer and inhibits anti-tumour immunityPubmed: 33041668
- Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brainPubmed: 32375811
- Association of IL-35 expression and gene polymorphisms in rheumatoid arthritis33307515
- Decreased interleukin-35 levels and CD4+ EBI3+ T cells in patients with type 1 diabetes and the effects of the antibody against CD20 (rituximab)33488880
- Anti-inflammatory roles of interleukin-35 in the pathogenesis of Japanese cedar pollinosis34386410
- Pentosan Polysulfate Sodium augments the therapeutic effect of 5-Aminosalicylic Acid in DSS colitis model; the role of IL-35 expressionPubmed:35247859
- Association of three single nucleotide polymorphisms of interleukin-35 for detection and prediction of women with breast cancer in Erbil city
- Analysis of IL-10 and IL-35 in DPP-4 inhibitor-related bullous pemphigoid