Recombinant Nephrin (NPHN) Homo sapiens (Human) Recombinant protein

NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Molecular Mass 41.7kDa, Accurate 42kDa(Analysis of differences refer to the manual)
  • Residues & TagsHis705~Ser1055 with N-terminal His Tag
  • Buffer Formulation100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point5.7
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  • Recombinant Nephrin (NPHN) Packages (Simulation)
  • Recombinant Nephrin (NPHN) Packages (Simulation)
  • Recombinant Nephrin (NPHN) Figure . Gene Sequencing (extract)
  • RPA937Hu03.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • The Level of Serum and Urinary Nephrin in Normal Pregnancy and Pregnancy with Subsequent Preeclampsia.pubmed:28120572
  • Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy.pubmed:29246788
  • Renal protection by atorvastatin in a murine model of sickle cell nephropathyPubmed:29527679
  • Multiplex Bead Array Assay of a Panel of Circulating Cytokines and Growth Factors in Patients with Albuminuric and Non-AlbuminuricDiabetic Kidney DiseasePubmed: 32961903
  • Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study34707724
  • Early biomarkers of nephrotoxicity associated with the use of anti-VEGF drugsPubmed:35620307

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