Recombinant Occludin (OCLN) Mus musculus (Mouse) Recombinant protein

Tight Junction Protein Occludin TM4 Minus

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Molecular Mass 33.8kDa, Accurate 42kDa(Analysis of differences refer to the manual)
  • Residues & TagsLys264~Pro521 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point5.7
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  • Recombinant Occludin (OCLN) Packages (Simulation)
  • Recombinant Occludin (OCLN) Packages (Simulation)
  • Recombinant Occludin (OCLN) Figure . Gene Sequencing (extract)
  • RPC228Mu02.png Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • HIV-1 Tat protein increases the permeability of brain endothelial cells by both inhibiting occludin expression and cleaving occludin via matrix metalloproteinase-9ScienceDirect: S0006899311021408
  • Disruption of the Circadian Clock in Mice Increases Intestinal Permeability and Promotes Alcohol-Induced Hepatic Pathology and InflammationPubMed: PMC3688973
  • A new panel of blood biomarkers for the diagnosis of mild traumatic brain injury/concussion in adultsPubMed: 25794137
  • Methods for Diagnosis and Treatment of Concussion or Brain Injury2016:0103140
  • Blood Occludin Level as a Potential Biomarker for Early Blood Brain Barrier Damage Following Ischemic Strokearticles:srep40331
  • Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal …Pubmed:29375374
  • Fas activation alters tight junction proteins in acute lung injuryPubmed: 30385692
  • The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned PigletsPubmed: 31426309
  • Peripheral blood occludin level as a biomarker for preoperative cerebral edema in patients with brain tumorsPubmed: 32884584
  • Intestinal Permeability in Participants With Thermal Injury: A Case Series From a Prospective, Longitudinal Study (HESTIA)
  • A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and …34492054
  • Higher serum occludin after successful reperfusion Is associated with early neurological deteriorationPubmed:35338575
  • Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation …

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