Recombinant Platelet Derived Growth Factor Subunit A (PDGFA) Homo sapiens (Human) Recombinant protein

PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 17.4kDa, Accurate 17kDa(Analysis of differences refer to the manual)
  • Residues & TagsSer87~Thr211 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point9.6
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  • Recombinant Platelet Derived Growth Factor Subunit A (PDGFA) Packages (Simulation)
  • Recombinant Platelet Derived Growth Factor Subunit A (PDGFA) Packages (Simulation)
  • Recombinant Platelet Derived Growth Factor Subunit A (PDGFA) Figure. Gene Sequencing (Extract)
  • RPA528Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy ratspubmed:28177680
  • Shikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathwaypubmed:28521243
  • Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells
  • Lipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathwayPubmed: 31698141
  • PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosisPubmed: 32208584
  • Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method34339025
  • Thermal Oscillation Changes the Liquid-Form Autologous Platelet-Rich Plasma into Paste-Like FormPubmed:35586817

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