Recombinant Platelet Factor 4 (PF4) Sus scrofa; Porcine (Pig) Recombinant protein

CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 19.0kDa, Accurate 22kDa(Analysis of differences refer to the manual)
  • Residues & TagsAsn54~Gln103 with Two N-terminal Tags, His-tag and SUMO-tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point9.1
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  • Recombinant Platelet Factor 4 (PF4) Packages (Simulation)
  • Recombinant Platelet Factor 4 (PF4) Packages (Simulation)
  • RPA172Po05.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic ratsPubMed: 20601223
  • Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilationPubMed: 22899170
  • Platelets Recognize Brain-Specific Glycolipid Structures, Respond to Neurovascular Damage and Promote NeuroinflammationPubMed: PMC3608633
  • Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompressionPubmed:25311322
  • A Phellinus baumii–based supplement containing Salvia miltiorrhiza Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathwaysin vitro and in vivoscience:S1756464616300676
  • Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injurypubmed:27583400
  • Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation.pubmed:28272338
  • Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation abilitypubmed:28450960
  • Aspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in ratsPubmed: 30528429
  • Short and long-term changes in platelet and inflammatory biomarkers after cryoballoon and radiofrequency ablationPubmed: 30857843
  • Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers
  • Newly Identified HNP‐F from Human Neutrophil Peptide 1 Promotes HemostasisPubmed: 30927490
  • Data for short and long-term prothrombotic biomarkers after cryoballoon and radiofrequency ablation
  • Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular MarkersPubmed: 32812629
  • The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in RodentsPubmed: 31533230
  • Feasibility study of use of rabbit blood to evaluate platelet activation by medical devicesPubmed: 31838449
  • The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets34099640
  • The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female ratsPubmed:35183388

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