Recombinant Preprooxytocin (OT) Homo sapiens (Human) Recombinant protein

OXT; Oxytocin,Prepropeptide; Oxytocin,Prepro-Neurophysin I; Oxytocin-Neurophysin 1

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 14.6kDa, Accurate 15kDa(Analysis of differences refer to the manual)
  • Residues & TagsCys20~Arg125 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose .
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point5.8
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  • Recombinant Preprooxytocin (OT) Packages (Simulation)
  • Recombinant Preprooxytocin (OT) Packages (Simulation)
  • RPD767Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Key roles of cyclooxygenase 2-protein kinase A-HCN3 pathway in the regulation of oxytocin neuronal activity in lactating rats with intermittent pup-deprivationPubmed: 33137408
  • Involvement of Protein Kinase A in Oxytocin Neuronal Activity in Rat Dams with Pup Deprivation33611682
  • Intranasal Oxytocin Restores Maternal Behavior and Oxytocin Neuronal Activity in the Supraoptic Nucleus in Rat Dams with Cesarean Delivery34166764

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