Recombinant Pyruvate Carboxylase (PC) Homo sapiens (Human) Recombinant protein

PCB

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMitochondrion
  • Molecular Mass 53.6kDa, Accurate 54/30kDa(Analysis of differences refer to the manual)
  • Residues & TagsPro36~Glu486 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.2
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  • Recombinant Pyruvate Carboxylase (PC) Packages (Simulation)
  • Recombinant Pyruvate Carboxylase (PC) Packages (Simulation)
  • Recombinant Pyruvate Carboxylase (PC) Figure . Gene Sequencing (extract)
  • RPH430Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Treadmill exercise rescues mitochondrial function and motor behavior in the CAG140 knock-in mouse model of Huntington's diseasePubmed: 31778667

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