Recombinant S100 Calcium Binding Protein A12 (S100A12) Bos taurus; Bovine (Cattle) Recombinant protein

CGRP; P6; CAAF1; CAGC; ENRAGE; MRP6; Calgranulin C; Calcium-binding protein in amniotic fluid 1; Extracellular newly identified RAGE-binding; Migration inhibitory factor-related 6

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Cytoplasm
  • Molecular Mass 14.2kDa, Accurate 14kDa(Analysis of differences refer to the manual)
  • Residues & TagsThr2~Glu92 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01%skl, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point5.9
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  • Recombinant S100 Calcium Binding Protein A12 (S100A12) Packages (Simulation)
  • Recombinant S100 Calcium Binding Protein A12 (S100A12) Packages (Simulation)
  • RPB080Bo01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Correlation of human S100A12 (EN-RAGE) and high-sensitivity C-reactive protein as gingival crevicular fluid and serum markers of inflammation in chronic periodontitis and type 2 diabetesPubmed: 24378957
  • The Circulating Level of Soluble Receptor for Advanced Glycation End Products Displays Different Patterns in Ulcerative Colitis and Crohn’s Disease: A Cross-Sectional StudyPubMed: 25757448
  • Low Endogenous Secretory Receptor for Advanced Glycation End-Products Levels Are Associated With Inflammation and Carotid Atherosclerosis in PrediabetesPubmed:26885882
  • High intake of dietary advanced glycation end-products is associated with increased arterial stiffness and inflammation in subjects with type 2 diabetes.pubmed:28958695
  • HbA1c Identifies Subjects With Prediabetes and Subclinical Left Ventricular Diastolic Dysfunction.pubmed:28973588
  • A Granulocyte-Specific Protein S100A12 as a Potential Prognostic Factor Affecting Aggressiveness of Therapy in Patients with Juvenile Idiopathic ArthritisDoi: 10.1155/2018/5349837
  • The role of serum S100A12 (calgranulin C) as a diagnostic marker in Egyptian patients with irritable bowel syndrome and ulcerative colitisDoi: 10.4103/AZMJ.AZMJ_52_18
  • Analysis of S100A12 plasma levels in hyperlipidemic subjects with or without familial hypercholesterolemiaPubmed: 30963307
  • 1 h Postload Glycemia Is Associated with Low Endogenous Secretory Receptor for Advanced Glycation End Product Levels and Early Markers of Cardiovascular …
  • Multimarker Approach to Identify Patients with Coronary Artery Disease at High Risk for Subsequent Cardiac Adverse Events: The Multi-Biomarker StudyPubmed: 32549327
  • Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris
  • The Serum and Saliva Proteome of Dogs with Diabetes Mellitus33271797

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