Recombinant Spondin 2 (SPON2) Mus musculus (Mouse) Recombinant protein

DIL1; Mindin; M-spondin; Extracellular Matrix Protein; Differentially expressed in cancerous and non-cancerous lung cells 1

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Extracellular matrix
  • Molecular Mass 26.7kDa, Accurate 33kDa(Analysis of differences refer to the manual)
  • Residues & TagsVal119~Val330 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point5.8
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  • Recombinant Spondin 2 (SPON2) Packages (Simulation)
  • Recombinant Spondin 2 (SPON2) Packages (Simulation)
  • Recombinant Spondin 2 (SPON2) Figure. Gene Sequencing (Extract)
  • RPF396Mu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Spondin-2, a secreted extracellular matrix protein, is a novel diagnostic biomarker for prostate cancerPubmed: 23665271
  • SP136EVALUATION OF SERUM SPONDIN 2 LEVELS IN THE DIFFERENT ETIOLOGIES OF GLOMERULAR DISEASESContent: 30
  • Evaluation of Serum Spondin 2 Levels in the Different Stages of Type 2 Diabetic NephropathyPubMed: 25973958
  • The extracellular matrix protein mindin attenuates colon cancer progression by blocking angiogenesis via Egr-1-mediated regulationpubmed:28991232
  • Study of human T21 placenta suggests a potential role of mesenchymal spondin-2 in placental vascular developmentPubmed: 30715257
  • Mindin serves as a tumour suppressor gene during colon cancer progression through MAPK/ERK signalling pathway in micePubmed: 32614521

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