Recombinant Tryptase (TPS) Homo sapiens (Human) Recombinant protein

TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 30.7kDa, Accurate 33kDa(Analysis of differences refer to the manual)
  • Residues & TagsIle31~Pro275 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.8
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  • Recombinant Tryptase (TPS) Packages (Simulation)
  • Recombinant Tryptase (TPS) Packages (Simulation)
  • RPB070Hu02.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polypsPubMed: 22534535
  • Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2Pubmed:24530123
  • Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis HerpetiformisPubmed:25400334
  • A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in RatsPubmed:25541997
  • Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgeryPubmed:25677271
  • Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary ReportPubMed: 26089601
  • Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitisPubMed: 26049029
  • Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell originsJournals: Mi
  • Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell OriginsPubmed:27057098
  • An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis
  • Dengue virus-elicited tryptase induces endothelial permeability and shockPubmed: 31265436
  • Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm FunctionPubmed: 31998139
  • Biomarkers of Allergic Asthma and their Association with Serum Parameters
  • Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity
  • Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive …34650557
  • Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia SyndromePubmed:35612327

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