Recombinant Tumor Necrosis Factor Beta (TNFb) Canis familiaris; Canine (Dog) Recombinant protein

LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Secreted
  • Molecular Mass 14.5kDa, Accurate 15kDa(Analysis of differences refer to the manual)
  • Residues & TagsLeu66~Asp185 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point8.2
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  • Recombinant Tumor Necrosis Factor Beta (TNFb) Packages (Simulation)
  • Recombinant Tumor Necrosis Factor Beta (TNFb) Packages (Simulation)
  • Recombinant Tumor Necrosis Factor Beta (TNFb) Figure . Gene Sequencing (extract)
  • RPA134Ca01.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling processPubMed: 22984252
  • Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantationPubmed:26847569
  • Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected CytokinesIJST-2328-3548-04-803
  • Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a …Pubmed:29305686
  • Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism …Pubmed: 32680553
  • Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort
  • Elevated lymphotoxin-¦Á (TNF¦Â) is associated with intervertebral disc degeneration33441130

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