Recombinant Tyrosine Hydroxylase (TH) Rattus norvegicus (Rat) Recombinant protein

TYH; DYT5b; Tyrosine 3-Monooxygenase; Dystonia 14

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 37.1kDa, Accurate 37kDa(Analysis of differences refer to the manual)
  • Residues & TagsPhe34~Leu301 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point6.2
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  • Recombinant Tyrosine Hydroxylase (TH) Packages (Simulation)
  • Recombinant Tyrosine Hydroxylase (TH) Packages (Simulation)
  • Recombinant Tyrosine Hydroxylase (TH) Figure . Gene Sequencing (extract)
  • RPB438Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • 1, 25-dyhydroxyvitamin D3 Attenuates l-DOPA-Induced Neurotoxicity in Neural Stem CellsPubmed:25102940
  • Genetic Diagnosis of Two Dopa-Responsive Dystonia Families by Exome SequencingPubmed:25181484
  • Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat.pubmed:28199883
  • Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population.pubmed:27540296
  • Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats.pubmed:28230187
  • Vaccarin administration ameliorates hypertension and cardiovascular remodeling in renovascular hypertensive ratspubmed:28681939
  • Multigenerational disruption of the thyroid endocrine system in marine medaka after a life-cycle exposure to perfluorobutanesulfonatePubmed:29565584
  • Cilostazol Mediated Nurr1 and Autophagy Enhancement: Neuroprotective Activity in Rat Rotenone PD ModelPubmed:29429051
  • Tetrabromobisphenol A caused neurodevelopmental toxicity via disrupting thyroid hormones in zebrafish larvaePubmed:29407805
  • The Effect of Recombinant Tyrosine Hydroxylase Expression on the Neurogenic Differentiation Potency of Mesenchymal Stem CellsPubmed:29656620
  • Montelukast attenuates rotenone-induced microglial activation/p38 MAPK expression in rats: Possible role of its antioxidant, anti-inflammatory and antiapoptotic effectsPubmed: 30222980
  • Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleusPubmed: 30581101
  • Hydroxychloroquine antiparkinsonian potential: Nurr1 modulation versus autophagy inhibition
  • Perfluoropolyether carboxylic acids (novel alternatives to PFOA) impair zebrafish posterior swim bladder development via thyroid hormone disruptionPubmed: 31733528
  • Embryonic exposure to pentabromobenzene inhibited the inflation of posterior swim bladder in zebrafish larvaePubmed: 31935612
  • Nose to brain delivery of rotigotine loaded chitosan nanoparticles in human SH-SY5Y neuroblastoma cells and animal model of Parkinson's diseasePubmed: 32084576
  • Thyroid disruption and developmental toxicity caused by Cd2+ in Schizopygopsis younghusbandi larvaePubmed: 32344129
  • Selection of Cells for Parkinson's Disease Cell-Therapy
  • Effects of SiO2 nanoparticles on the uptake of tetrabromobisphenol A and its impact on the thyroid endocrine system in zebrafish larvae33385677
  • Tocotrienols protect differentiated SH-SY5Y human neuroblastoma cells against 6-hydroxydopamine-induced cytotoxicity by ameliorating dopamine biosynthesis and …Pubmed:35065349
  • Omarigliptin attenuates rotenone-induced Parkinson's disease in rats: Possible role of oxidative stress, endoplasmic reticulum stress and immune modulationPubmed:35439590
  • Unravelling the neuroprotective mechanisms of carotenes in differentiated human neural cells: biochemical and proteomic approachesPubmed:35415676
  • Parental and transgenerational impairments of thyroid endocrine system in zebrafish by 2, 4, 6-tribromophenol

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