Recombinant Wingless Type MMTV Integration Site Family, Member 1 (WNT1) Homo sapiens (Human) Recombinant protein

INT1; Proto-oncogene Int-1 homolog

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 68kDa, Accurate 66kDa(Analysis of differences refer to the manual)
  • Residues & TagsAla28~Leu370 with N-terminal His and GST Tag
  • Buffer Formulation100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point9.2
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  • Recombinant Wingless Type MMTV Integration Site Family, Member 1 (WNT1) Packages (Simulation)
  • Recombinant Wingless Type MMTV Integration Site Family, Member 1 (WNT1) Packages (Simulation)
  • RPL821Hu01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Docosahexaenoic acid inhibits 12-O-tetradecanoylphorbol-13- acetate-induced fascin-1-dependent breast cancer cell migration by suppressing the PKCδ- and Wnt-1/β-catenin-mediated pathwaysPubmed:27036017
  • Wnt1 inhibits vascular smooth muscle cell calcification by promoting ANKH expressionPubmed: 31356809

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