Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) Rattus norvegicus (Rat) Recombinant protein

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Extracellular matrix
  • Molecular Mass 39.5kDa, Accurate 40kDa(Analysis of differences refer to the manual)
  • Residues & TagsIle62~Lys380 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 97%
  • Isoelectric Point8.8
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  • Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) Packages (Simulation)
  • Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) Packages (Simulation)
  • Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) Figure . Gene Sequencing (extract)
  • RPP549Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosisPubmed:25488180
  • Wnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cellsPubMed: 26547443
  • Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosisPubMed: 25488180
  • Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell culturesPubMed: 26163991
  • Untersuchungen der Serum-Konzentrationen des proinflammatorischen Glykoproteins wnt-5a und seines antiinflammatorischen Inhibitors sFRP-5 bei an …servlets:MCRFileNodeServlet
  • Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways.pubmed:28754618
  • Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With …Pubmed:29674475
  • Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis‐Supportive Function of Fanconi Anemia Mesenchymal Stromal CellsPubmed:29377497
  • Adipose tissue–derived WNT5A regulates vascular redox signaling in obesity via USP17/RAC1-mediated activation of NADPH oxidasesPubmed: 31534019
  • Role of wnt5a in Metabolic Inflammation in HumansPubmed: 30137542
  • Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer MetastasisPubmed: 31932454
  • Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive diseasePubmed: 33188773
  • Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and …Pubmed: 32491086
  • An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties34823885
  • Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort studyPubmed:34990470
  • Association between bone turnover markers and periodontitis: A population‐based cross‐sectional studyPubmed:35569034

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