ELISA Kit DIY Materials for Beta Actin (ACTB) Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Assay Kit DIY Materials

Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.57ng/mL
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  • ELISA Kit DIY Materials for Beta Actin (ACTB) Packages (Simulation)
  • ELISA Kit DIY Materials for Beta Actin (ACTB) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Actin Beta (ACTb).No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral MusclesNCBI:PMC3856104
  • Expression of chloride channels in mouse skin incisional wounds96
  • Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes
  • Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo MedicinePubmed: 31902915
  • Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor …Pubmed: 32046779
  • Effect of ischemic postconditioning on cell apoptosis and expression of relevant genes in non-culprit coronary arteriesPubmed: 32784207
  • Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring
  • Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells33261077
  • POLRMT mutations impair mitochondrial transcription causing neurological disease33602924
  • Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexusPubmed:34990649
  • Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn.Pubmed:35398498
  • Investigation of single beam ultrasound sensitivity as a monitoring tool for local hyperthermia treatment in breast cancer

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