ELISA Kit DIY Materials for Amphiregulin (AREG) Homo sapiens (Human) Assay Kit DIY Materials

AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1,000pg/mL
  • Applicable Sensitivity6.5pg/mL
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  • ELISA Kit DIY Materials for Amphiregulin (AREG) Packages (Simulation)
  • ELISA Kit DIY Materials for Amphiregulin (AREG) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Amphiregulin (AREG).No significant cross-reactivity or interference between Amphiregulin (AREG) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Amphiregulin and Epiregulin Expression in Colorectal Carcinoma and the Correlation with Clinicopathological CharacteristicsKarger: 315380
  • Serum levels of hepatocyte growth factor and epiregulin are associated with the prognosis on anti-EGFR antibody treatment in KRAS wild-type metastatic colorectal cancer.Pubmed:24800946
  • Association between serum ligands and the skin toxicity of anti-epidermal growth factor receptor antibody in metastatic colorectal cancerPubMed: 25707609
  • Amphiregulin confers trastuzumab resistance via AKT and ERK activation in HER2-positive breast cancerPubMed: 26195282
  • Serum level of hepatocyte growth factor is a novel marker of predicting theoutcome and resistance to the treatment with trastuzumab in HER2-positive patients with metastatic gastric cancerPubmed:26716644
  • Anti-asthmatic activity of alkaloid compounds from Pericarpium Citri Reticulatae (Citrus reticulata 'Chachi')Pubmed: 19363516
  • CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17
  • Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicinePubmed: 33212364
  • Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheetsPubmed: 33164313

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