ELISA Kit DIY Materials for Antithrombin (AT)
AT3; ATIII; SERPINC1; Anti-Thrombin Antibodies; Serpin Peptidase Inhibitor Clade C Member 1; Coding Sequence Signal Peptide Antithrombin Part 1
- UOM
- FOB US$ 1,296.00 US$ 2,160.00 US$ 3,888.00 US$ 7,560.00 US$ 12,960.00
- Quantity
Overview
Properties
- Product No.KSC313Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryEnzyme & KinaseMetabolic pathwayHematology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.94-60ng/mL
- Applicable Sensitivity0.42ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Antithrombin (AT).No significant cross-reactivity or interference between Antithrombin (AT) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Thalidomide and multiple myeloma serum synergistically induce a hemostatic imbalance in endothelial cells in vitroPubMed: 25840743
- iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Managementpubmed:29247448
- Detection of Angiotensin II and AT1 Receptor Concentrations in Keloid and Hypertrophic Scar.
- An Agile Approach to Novel Recombinant Antithrombin Production for use in Biologics Development