ELISA Kit DIY Materials for Apolipoprotein A4 (APOA4) Mus musculus (Mouse) Assay Kit DIY Materials

ApoA-IV; ApoAIV; Apo-A4

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.69ng/mL
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  • ELISA Kit DIY Materials for Apolipoprotein A4 (APOA4) Packages (Simulation)
  • ELISA Kit DIY Materials for Apolipoprotein A4 (APOA4) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Apolipoprotein A4 (APOA4).No significant cross-reactivity or interference between Apolipoprotein A4 (APOA4) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant propertiesPubMed: 23328279
  • Differential plasma proteome analysis in patients with high?altitude pulmonary edema at the acute and recovery phasesPubmed:24940404
  • Human maternal plasma proteomic changes with parturitionSciencedirect:S2212968514000506
  • Development of concise audit standards for unplanned return to theate following gynaecological surgery at Gloucestershire Royal HospitalDoi: 10.1111
  • Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2JPubmed: 32585848
  • Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and CatsPubmed: 32880234
  • Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer33602229

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