ELISA Kit DIY Materials for Arginase (ARG) Mus musculus (Mouse) Assay Kit DIY Materials

ARG1; Arginase I; Liver Arginase

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.056ng/mL
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  • ELISA Kit DIY Materials for Arginase (ARG) Packages (Simulation)
  • ELISA Kit DIY Materials for Arginase (ARG) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Arginase (ARG).No significant cross-reactivity or interference between Arginase (ARG) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Phenobarbital Induces Alterations in the Proteome of Hepatocytes and Mesenchymal Cells of Rat LiversPubmed: 24204595
  • Arginase I release from activated neutrophils induces peripheral immunosuppression in a murine model of strokePubMed: 25966956
  • Circulating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host diseasePubmed:27109254
  • Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonicaPubmed:27157496
  • Potent but transient immunosuppression of T-cells is a general feature of erythroid progenitor cells
  • Potent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells34893694

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