ELISA Kit DIY Materials for Beta-2-Microglobulin (b2M)

BMG; B2MG

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 720.00 US$ 1,200.00 US$ 2,160.00 US$ 4,200.00 US$ 7,200.00
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Overview

Properties

Product No.KSA260Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
DownloadInstruction Manual
CategoryInfection immunity Kidney biomarker

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range0.78-50ng/m
Applicable Sensitivity0.29ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Beta-2-Microglobulin (b2M). No significant cross-reactivity or interference between Beta-2-Microglobulin (b2M) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

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Citations

The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary StudiesHindawi: 740105
Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recoveryBiomedcentral: Source
Mef2C restrains microglial inflammatory response and is lost in brain ageing in an IFN-I-dependent manner.pubmed:28959042
Urine proteome analysis by C18 plate–matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis …Pubmed:30024955
Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approachPubmed:29315472
Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar ratsPubmed: 30426860
Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese PatientsPubmed: 30486327
iTRAQ-based analysis for the identification of MARCH8 targets in human esophageal squamous cell carcinoma33540066
Diminishment of Nrf2 Antioxidative Defense Aggravates Nephrotoxicity of Melamine and Oxalate Coexposure. Antioxidants 2021, 10, 146434573096
Biomarkers of cadmium exposure and renal function in estuarine adult villagers34773507