ELISA Kit DIY Materials for C Reactive Protein (CRP) Homo sapiens (Human) Assay Kit DIY Materials

C-RP; PTX1; Pentraxin-Related; Pentraxin 1

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range62.5-4,000pg/mL
  • Applicable Sensitivity26.1pg/mL
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  • ELISA Kit DIY Materials for C Reactive Protein (CRP) Packages (Simulation)
  • ELISA Kit DIY Materials for C Reactive Protein (CRP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of C Reactive Protein (CRP). No significant cross-reactivity or interference between C Reactive Protein (CRP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Effect of Hirulog-Like Peptide on Balloon Catheter Injury-Induced Neointimal Formation in Femoral Arteries of Minipigs and Relationship with Inflammatory MediatorsKarger: 000257340
  • Successful Treatment of Acute Lung Injury with Pitavastatin in Septic Mice: Potential Role of Glucocorticoid Receptor Expression in Alveolar MacrophagesAspet: 171462
  • Anti-Inflammatory Effect of Guan-Xin-Er-Hao via the Nuclear Factor-Kappa B Signaling Pathway in Rats with Acute Myocardial InfarctionJstage: source
  • In vivo protective effect of ferulic acid against noise-induced hearing loss in the guinea-pigPubMed: 20600667
  • Metformin inhibits nuclear factor κB activation and decreases serum high-sensitivity C-reactive protein level in experimental atherogenesis of rabbitsSpringerLink: pt732478620w1528
  • Olive (Olea europaea L.) Leaf Polyphenols Improve Insulin Sensitivity in Middle-Aged Overweight Men: A Randomized, Placebo-Controlled, Crossover TrialPubMed: 23516412
  • Unpredictable chronic mild stress promotes atherosclerosis in high cholesterol-fed rabbitsPubMed: 22753638
  • Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in MicePlosOne: Source
  • Relationships among serum C-reactive protein, receptor for advanced glycation products, metabolic dysfunction, and cognitive impairmentsPubMed: PMC3765839
  • Impact of experimental type 1 diabetes mellitus on systemic and coagulation vulnerability in mice acutely exposed to diesel exhaust particlesPubMed: PMC3641025
  • Changes in body weight, C-reactive protein, and total adiponectin in non-obese women after 12 months of a small-volume, home-based exercise programPubMed: PMC3752622
  • Pre-Pubertal Children Born Post-Term Have Reduced Insulin Sensitivity and Other Markers of the Metabolic SyndromePubMed: PMC3698136
  • Among overweight middle-aged men, first-borns have lower insulin sensitivity than second-bornsPubmed: 24503677
  • Perirenal fat associated with microalbuminuria in obese ratsPubmed: 24526332
  • Effect of caffeic acid on doxorubicin induced cardiotoxicity in ratsAjbm: Source
  • Effect of high free fatty acids on the anti-contractile response of perivascular adipose tissue in rat aortaPubmed: 23939490
  • Flos Lonicera Ameliorates Obesity and Associated Endotoxemia in Rats through Modulation of Gut Permeability and Intestinal MicrobiotaPlos: Source
  • The determination of histopathological and biochemical effects of the rabbit knee joint injected dexketoprofen trometamolOnlinelibrary: fcp.12074
  • Identifizierung neuer potentieller Biomarker für das Kolonkarzinom sowie dessen VorstufenInfo:Source
  • Celastrol may have an anti-atherosclerosis effect in a rabbit experimental carotid atherosclerosis modelPubmed:Pmc4132129
  • As If Biomarker Discovery Isn't Hard Enough: The Consequences of Poorly Characterized ReagentsPubmed:24305687
  • Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbitsPubmed:25282128
  • Regression of experimental endometriotic implants in a rat model with the angiotensin II receptor blocker losartanPubmed:25302540
  • Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal InstillationPubmed:25580880
  • Bisphenol A exposure induces metabolic disorders and enhances atherosclerosis in hyperlipidemic rabbitsPubmed:25619500
  • Short-Term Nose-Only Water-Pipe (Shisha) Smoking Exposure Accelerates Coagulation and Causes Cardiac Inflammation and Oxidative Stress in MicePubmed:25634761
  • Consumption of orange fermented beverage reduces cardiovascular risk factors in healthy micePubmed:25666657
  • Induction of Haemeoxygenase-1 Improves FFA-Induced Endothelial Dysfunction in Rat AortaPubmed:25766533
  • 巨噬细胞及其相关因子在家兔不稳定斑块中的作用及其机制Paper:Source
  • Effects of Intravenous Injection ofPubMed: 26063970
  • Irisin improves endothelial function in obese mice through the AMPK-eNOS pathwayPubMed: 26371167
  • Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos PathwayPubMed: 26160485
  • Renal protective effects of induction of haem oxygenase-1 combined with increased adiponectin on the glomerular vascular endothelial growth factor–nitric oxide axis in obese ratsPubMed: 25959017
  • Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs?PubMed: 26556576
  • Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal InstillationPubMed: 25580880
  • Resveratrol Inhibited Inflammation andAlveolar Bone Loss in PeriodontitisPubMed: 26497626
  • Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorderPubmed:26934364
  • Orange beverage ameliorates high-fat-diet-induced metabolic disorder in micearticle:S1756464616300688
  • Variation of Circulating Inflammatory Mediators in Staphylococcus aureus and Escherichia coli BloodstreamInfection.Pubmed:26772168
  • Central Administration of Galanin Receptor 1 Agonist Boosted Insulin Sensitivity in Adipose Cells of Diabetic Ratspmc:PMC4835658
  • THE ANTI-HYPERCHOLESTEROLEMIC EFFECT OF ULVAN POLYSACCHARIDE EXTRACTED FROM THE GREEN ALGA ULVAFASCIATA ON AGED HYPERCHOLESTEROLEMIC RATSjournals:10835
  • Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult RatsPubmed:26175082
  • Combined oral contraceptive-induced hypertension is accompanied by endothelial dysfunction and upregulated intrarenal angiotensin II type 1 receptor gene expressionpubmed:27447455
  • Cardioprotective and renoprotective effects of Cocos nucifera water in offspring of high fat diet fed Wistar rat damsqk:20168
  • Eicosapentaenoic acid-enriched phospholipids improve atherosclerosis by mediating cholesterol metabolismS1756464617300890
  • Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats.pubmed:28376377
  • Chronic prostatitis/chronic pelvic pain syndrome impairs erectile function through increased endothelial dysfunction, oxidative stress, apoptosis, and corporal fibrosis …doi:10.1111
  • Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adiposepubmed:27127795
  • Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population.pubmed:27540296
  • Acute phase response in lame crossbred dairy cattlepubmed:27956769
  • Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis.pubmed:27880884
  • INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATIONpubmed:27984536
  • PROCALCITONIN, C-REACTIVE PROTEIN AND FIBRINOGEN LEVELS IN CASES WITH MECHANIC BOWEL OBSTRUCTION AND THEIR CHANGE IN RELATION TO THE DURATION OF OBSTRUCTION: AN EXPERIMENTAL STUDY10.3892/ol.2017.7301
  • Relationship of mean platelet volume to lymphocyte ratio and coronary collateral circulation in patients with stable angina pectorispubmed:28678144
  • Protective effect of α–lipoic acid against spleen toxicity of dimethylnitrosamine in male mice: Antioxidant and ultrastructure approachespubmed:29031205
  • The effects of non-surgical periodontal treatment on glycemic control, oxidative stress balance and quality of life in patients with type 2 diabetes: A randomized clinical trialpubmed:29145468
  • The effect of DL-, L-isomers and DL-hydroxy analog administered at 2 levels as dietary sources of methionine on the metabolic and antioxidant parameters and growth performance of turkeys10.3382/ps/pex099
  • The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis.pubmed:29029533
  • Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women10.1590/MEDICC.2017.1902030004 
  • The relationship between anti-C-reactive protein and disease activity in patients with systemic lupus erythematosus.pubmed:28352058
  • Grape Seed Procyanidin Extract Reduces Arsenic-Induced Renal Inflammatory Injury in Male Mice.pubmed:28756814
  • Anti-inflammatory and antithrombotic effects of nicotine exposure in oral contraceptive-induced insulin resistance are glucocorticoid-independentpubmed:28349880
  • A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defensePubmed:29410422
  • Leptin alleviates intestinal mucosal barrier injury and inflammation in obese mice with acute pancreatitisPubmed:29934609
  • The Protective Activities of Dietary Sea Cucumber Cerebrosides Against Atherosclerosis Through Regulating Inflammation and Cholesterol Metabolism in Male MicePubmed:29883529
  • Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague …Pubmed:29849871
  • L-Carnitine Supplementation in Older Women. A Pilot Study on Aging Skeletal Muscle Mass and FunctionPubmed:29473908
  • Saponins of sea cucumber attenuate atherosclerosis in ApoE−/− mice via lipid-lowering and anti-inflammatory properties10.1016:j.jff.2018.07.046
  • Generation and validation of novel anti-bovine CD163 monoclonal antibodies ABM-1A9 and ABM-2D6Pubmed:29571519
  • Oxymatrine attenuates lipopolysaccharide-induced acute lung injury by activating the epithelial sodium channel and suppressing the JNK signaling pathwayPubmed:29526865
  • Anti-Inflammatory Activity of Combined Aerodisperse System in the Conditions of Experimental Cervical Lymphadenite:
  • Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromePubmed: 30342783
  • Sonographic synovitis in primary knee osteoarthritis patients and its relation to biomarkers of inflammation and oxidative stressDoi: 10.1016/j.ejr.2018.12.004
  • Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.
  • L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged WomenPubmed: 30485835
  • The Egyptian Rheumatologist
  • Grape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signalingPubmed: 30869553
  • Morphological Characteristics of the Thymus and Spleen and the Subpopulation Composition of Lymphocytes in Peripheral Blood during Systemic Inflammatory …
  • Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry
  • Periodontal Health and Oral Microbiota in Patients with Rheumatoid ArthritisPubmed: 31072030
  • Effects of Eurycoma longifolia Jack on chronic cerebral hypoperfusion-induced oxidative damage and memory deficit in rats
  • Eicosapentaenoic acid in the form of phospholipid exerts superior anti-atherosclerosis effects than its triglyceride form in ApoE-/-mice
  • Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry/Nova metoda detekcie CRP proteinu na kralicich leukocytoch pomocou …
  • The potential efficacy of dietary fatty acids and fructose induced inflammation and oxidative stress on the insulin signaling and fat accumulation in micePubmed: 31672515
  • Ginsenoside Rg3-loaded, reactive oxygen species-responsive polymeric nanoparticles for alleviating myocardial ischemia-reperfusion injuryPubmed: 31783047
  • Rotundic Acid Protects against Metabolic Disturbance and Improves Gut Microbiota in Type 2 Diabetes RatsPubmed: 31887996
  • Lowering effects of fish oil supplementation on proinflammatory markers in hypertension: results from a randomized controlled trialPubmed: 32044905
  • Silica nanoparticles induce JNK-mediated inflammation and myocardial contractile dysfunctionPubmed: 32036317
  • The Efficacy of Autologous Myoblast Sheet Transplantation to Prevent Perforation After Duodenal Endoscopic Submucosal Dissection in Porcine ModelPubmed: 33121270
  • miR-205/IRAK2 signaling pathway is associated with urban airborne PM2.5-induced myocardial toxicityPubmed: 32880512
  • Protective Effects of Statin and Angiotensin Receptor Blocker in a Rat Model of Doxorubicin-and Trastuzumab-Induced CardiomyopathyPubmed: 32778498
  • Association between Plasma Sestrin2 Levels and the Presence and Severity of Coronary Artery DiseasePubmed: 32670435
  • Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in ratsPubmed: 32616735
  • Quantifying the C-reactive protein concentrations of uterine lavage samples in postpartum dairy cowsPubmed: 32408965
  • Serum levels of olfactomedin 4: a biomarker for asthma control state in asthmaticsPubmed: 32395538
  • Validation and adaptation of the Empirical Dietary Inflammatory Pattern (EDIP) across nations: a test case.Pubmed: 32563770
  • Effects of Tempol in Lipopolysaccharide-Induced Liver Injury
  • Possible Role of Statins on the Inflammatory Biomarkers in Patients With Periodontal Disease: A Cross-Sectional Study
  • Endothelin-1 is a surrogate biomarker link severe periodontitis and endothelial dysfunction in hypertensive patients: The potential nexus
  • Effect of claw disorders on haemato-biochemical parameters and acute phase protein levels in crossbred cattle
  • Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles
  • Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes
  • МАРКЕРЫ КОСТНОГО РЕМОДЕЛИРОВАНИЯ ПРИ ЗАМЕЩЕНИИ ДЕФЕКТАТРАБЕКУЛЯРНОЙ КОСТНОЙ ТКАНИ РЕЗОРБИРУЕМЫМИ И …
  • Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans33407555
  • Use of Fourier-Transform Infrared Spectroscopy (FT-IR) for Monitoring Experimental Helicobacter pylori Infection and Related Inflammatory Response in Guinea Pig?¡­33396581
  • Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats33833309
  • Baicalin relieves Mycoplasma pneumoniae infection?induced lung injury through regulating microRNA?221 to inhibit the TLR4/NF?¦ÊB signaling pathway34109422
  • Prevalence and Factors Associated with Iron Deficiency and Anemia among Residents of Urban Areas of S?o Paulo, Brazil34072813
  • Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats33510972
  • Intravenous ferric carboxymaltose does not provide benefits in reperfused acute myocardial infarction in the rat with normal iron status34243097
  • Pim-2 kinase inhibits inflammation by suppressing the mTORC1 pathway in atherosclerosis34547720
  • Nets of biotin-derived gold nanoparticles as a label for the C-reactive protein immunoassay34559243
  • Cardio-protective and Anti-atherosclerosis Effect of Crocetin on Vitamin D3 and HFD-induced Atherosclerosis in Rats34615830
  • Early-stage serum Stanniocalcin 1 as a predictor of outcome in patients with aneurysmal subarachnoid hemorrhagePubmed:34941085
  • A rapid fluorescent aptasensor for point-of-care detection of C-reactive proteinPubmed:35714415
  • Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosisPubmed:35126715
  • A Two-step Competition Assay for Visual, Sensitive and Quantitative C-reactive Protein Detection in Low-cost Microfluidic Particle Accumulators

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