ELISA Kit DIY Materials for Carcinoembryonic Antigen (CEA) Homo sapiens (Human) Assay Kit DIY Materials

CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1,000pg/mL
  • Applicable Sensitivity6.5pg/mL
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  • ELISA Kit DIY Materials for Carcinoembryonic Antigen (CEA) Packages (Simulation)
  • ELISA Kit DIY Materials for Carcinoembryonic Antigen (CEA) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Carcinoembryonic Antigen (CEA). No significant cross-reactivity or interference between Carcinoembryonic Antigen (CEA) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis modelPubMed: 20872761
  • Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liverScienceDirect: S0024320511002700
  • Saffron Aqueous Extract Inhibits the Chemically-induced Gastric Cancer Progression in the Wistar Albino RatPubMed: PMC3637902
  • Handy, rapid and multiplex detection of tumor markers based on encoded silica-hydrogel hybrid beads array chipPubmed: 23672876
  • Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention.Pubmed:25684529
  • Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometryRsc:Source
  • Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive IndicatorPubMed: 26446295
  • Gene expression analysis in the lung of the rasH2 transgenic mouse at week 4 prior to induction of malignant tumor formation by urethane and N-methylolacrylamidePubMed: 26558449
  • Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human StudyPubmed:26934615
  • Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats2071_pdf.pdf
  • Temporal Sensing Platform Based on Bipolar Electrode for the Ultrasensitive Detection of Cancer Cellspubmed:27506255
  • Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in ratsv04n07a07a
  • Ultrasensitive cathode photoelectrochemical immunoassay based on TiO2 photoanode-enhanced 3D Cu2O nanowire array photocathode and signal amplification by …Pubmed:29866267
  • Evaluation of Detection Methods and Values of Circulating Vascular Endothelial Growth Factor in Lung CancerPubmed:29675110
  • 8-Hydroxy-2'-deoxyguanosine as a Discriminatory Biomarker For Early Detection of Breast Cancer
  • Preventive potential of dietary inclusion of Brachystegia eurycoma (Harms) seeds on N-methyl-N-nitrosourea-induced colon carcinogenesis in Wistar ratsPubmed: 30953819
  • Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma
  • DNA-templated porous nanoplatform towards programmed “double-hit” cancer therapy via hyperthermia and immunogenicity activationPubmed: 31374481
  • Piperine Regulates Nrf-2/Keap-1 Signalling and Exhibits Anticancer Effect in Experimental Colon Carcinogenesis in Wistar RatsPubmed: 32967203
  • Evaluation of the Diagnostic and Predicative Values of 8-Iso-Prostaglandin F2α as a Biomarker of Breast CancerPubmed: 32721979
  • Chemopreventive effect of dietary inclusion with Crassocephalum rubens (Juss ex Jacq) leaf on N-methyl-N-nitrosourea (MNU)-induced colorectal carcinogenesis in …
  • Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles
  • Anti-Cancer Activity of Piperine Against Colon Carcinogenesis Via Modulation of NF-κB/Nrf-2/Keap1/HO-1 Signalling Pathways
  • Magnetic immunoassay for tumor clinical diagnosis based on rolling circular amplification (RCA) coupled with ICP-MS
  • Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo
  • A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate

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