ELISA Kit DIY Materials for Caspase 1 (CASP1)
ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase
- UOM
- FOB US$ 1,197.00 US$ 1,995.00 US$ 3,591.00 US$ 6,983.00 US$ 11,970.00
- Quantity
Overview
Properties
- Product No.KSB592Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategorySignal transductionEnzyme & KinaseApoptosisInfection immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates, cell lysates , cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.118ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Caspase 1 (CASP1). No significant cross-reactivity or interference between Caspase 1 (CASP1) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-ReperfusionPubmed:24816434
- Inflammosome in the human endometrium: further step in the evaluation of the “maternal side”PubMed: 26474737
- Synthetic PreImplantation Factor (PIF) prevents fetal loss by modulating LPS induced inflammatory response.pubmed:28704412
- NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitinationPubmed:29386291
- Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation?Pubmed:29665864
- A role for the inflammasome in spontaneous labor at termPubmed:27852921
- CHARACTERIZATION OF PORCINE NLRP3 INFLAMMASOME-MEDIATED INTERLEUKIN-1 BETA PRODUCTION IN RESPONSE TO INFLUENZA A VIRUSESPubmed: 29386291
- NLRP3 inflammasome expression in lipopolysaccharide-induced otitis mediaPubmed: 30676848
- Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss womenPubmed: 31148259
- Serum Caspase-1 levels in women with polycystic ovary syndromePubmed: 32127139
- Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients
- ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES
- CLINICAL AND PATHOGENETIC SIGNIFICANCE OF CHANGES IN PURINE METABOLISM DURING LUNG CANCER
- The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation34560082
- The alarmin S100A12 causes sterile inflammation of the human chorioamniotic membranes and preterm birth and neonatal mortality in mice34632484
- TRPV1 Suppressed NLRP3 Through Regulating Autophagy in Microglia After Ischemia-Reperfusion InjuryPubmed:35041191
- Influenza A Virus Infection Activates NLRP3 Inflammasome through Trans-Golgi Network DispersionPubmed:35062292