ELISA Kit DIY Materials for Cathelicidin Antimicrobial Peptide (CAMP) Mus musculus (Mouse) Assay Kit DIY Materials

CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.78-50ng/mL
  • Applicable Sensitivity0.32ng/mL
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  • ELISA Kit DIY Materials for Cathelicidin Antimicrobial Peptide (CAMP) Packages (Simulation)
  • ELISA Kit DIY Materials for Cathelicidin Antimicrobial Peptide (CAMP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Cathelicidin Antimicrobial Peptide (CAMP). No significant cross-reactivity or interference between Cathelicidin Antimicrobial Peptide (CAMP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in BronchiectasisWaikato:Source
  • Host immune response to tuberculous meningitisPubmed:25301213
  • On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experimentPubmed:25444792
  • Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells Pubmed:25681250
  • Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer CellsPubMed: 25681250
  • Cathelicidin as a link between sarcoidosis and tuberculosisPubMed: 26422567
  • The association of vitamin D, cathelicidin, and vitamin D binding protein with acute asthma attacks in childrenPubMed: 26108071
  • Swiftly Decreasing Cerebrospinal Fluid Cathelicidin Concentration Predicts Improved Outcome in Childhood Bacterial Meningitis.Pubmed:27008883
  • Light-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin.pubmed:27315464
  • Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated withActivation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension.pubmed:27391973
  • Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance.pubmed:28261091
  • The relation of innate and adaptive immunity with viral-induced acute asthma attacks: Focusing on IP-10 and cathelicidinpubmed:27955890
  • Effects of cold exposure on the bone marrow adipose tissueDOI: 10.3969/j.issn.1674-2591.2017.05.008
  • Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping techniquePubmed:29374283
  • Antimalarial activity of vitamin D3 (VD3) does not result from VD3-induced antimicrobial agents including nitric oxide or cathelicidinPubmed: 30904694
  • Myeloid cell-derived LL-37 promotes lung cancer growth by activating Wnt/β-catenin signalingPubmed: 31149039
  • Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37Pubmed: 32050475
  • Hypovitaminosis D and reduced cathelicidin are strongly correlated during the multidrug therapy against leprosyPubmed: 32645421
  • Effect of Lonicerae japonicae Flos Carbonisata-Derived Carbon Dots on Rat Models of Fever and Hypothermia Induced by LipopolysaccharidePubmed: 32606669
  • Relações causais entre a expressão dos genes do receptor de Vitamina D e do Peptídeo Antimicrobiano Catelicidina sobre marcadores sorológicos de pessoas com …
  • Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies33999928
  • Analysis of the level of non-specific and specific immunity parameters in saliva of children with osteogenesis imperfecta and study of relationships between?¡­
  • Type I interferons link skin-associated dysbiotic commensal bacteria to pathogenic inflammation and angiogenesis in rosacea
  • Compartmentalized Innate Immune Response of Human Fetal Membranes against Escherichia coli Choriodecidual InfectionPubmed:35328414
  • LL-37 transports immunoreactive cGAMP to activate STING signaling and enhance interferon-mediated host antiviral immunityPubmed:35649354

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