ELISA Kit DIY Materials for Chemerin (CHEM) Mus musculus (Mouse) Assay Kit DIY Materials

TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.68ng/mL
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  • ELISA Kit DIY Materials for Chemerin (CHEM) Packages (Simulation)
  • ELISA Kit DIY Materials for Chemerin (CHEM) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Chemerin (CHEM). No significant cross-reactivity or interference between Chemerin (CHEM) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Serum chemerin and vaspin in non-alcoholic fatty liver diseasePubMed: 20095887
  • Chemerin, vaspin and insulin resistance in chronic hepatitis CWiley: source
  • Expression and regulation of chemerin during rat pregnancyScienceDirect: S0143400412000902
  • Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and ratsNature: 201211
  • Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnciRaeddergisi: Source
  • Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patientsPubmed: 24702860
  • Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjectsPubmed: 24303851
  • Impact of OSA on Biological Markers in Morbid Obesity and Metabolic SyndromePubmed:24106991
  • Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsiaPubmed:24840719
  • Relationship between plasma chemerin levels and disease severity in COPD patientsPubmed:24865134
  • Elevated serum chemerin in Chinese women with hyperandrogenic PCOSPubmed:24927079
  • Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats.Pubmed:25471281
  • Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty RatsPubmed:25697126
  • Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgarisSciencedirect:S1027811714000470
  • Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control StudyPubMed: 26170530
  • Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese PopulationPubMed: 26471865
  • Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant ratsPubMed: 25471281
  • Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytesPubmed:26721419
  • Association of chemerin levels and bone mineral density in Chinese obese postmenopausalwomen.pubmed:27583869
  • Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague …Pubmed:29849871
  • Chemerin is associated with diabetic retinopathy in type 2 diabetesPubmed:25848840
  • Expression of chemerin in the synovial fluid of patients with temporomandibular joint disordersPubmed:29331039
  • Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancerPubmed: 30252132
  • The decline of whole-body glucose metabolism in ovariectomized ratsPubmed: 30292771
  • Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-κB signalDoi: 10.1016/j.pan.2019.02.005
  • Protective effect of vitamin D supplementation in a rat modal of preeclampsia: a possible implication of chemerinPubmed: 30922121
  • Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-kB signalPubmed: 30833212
  • Association between High Levels of Circulating Chemerin and Colorectal Adenoma in MenPubmed: 31269491
  • Vitamin D Supplementation Reduces Serum Chemerin Level in Gestational Diabetes Mellitus Rat Model
  • Plasma levels of adiponectin and chemerin are associated with early stage of atherosclerosis in older adults with type 2 diabetes mellitus
  • The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren33575861
  • Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren33834274
  • Altered Serum Marker of Adipokines Profile in Breast Cancer Women
  • Association of High Serum Chemerin with Bone Mineral Density Loss and Osteoporotic Fracture in Elderly Chinese WomenPubmed:35140527

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